Central composite design optimization of active and physical properties of food packaging films based on chitosan/gelatin/pomegranate peel extract

Author(s)

Bertolo, Mirella Romanelli Vicente ; Dias, Lucas Danilo ; Filho, Josemar Goncalves de Oliveira ; Alves, Fernanda ; Marangon, Crisiane Aparecida ; Amaro Martins, Virginia da Conceica ; Ferreira, Marcos David ; Bagnato, Vanderlei Salvador ; Plepis, Ana Maria de Guzzi ; Bogustz Junior, Stanislau

• Document type: Journal article
• Source: FOOD PACKAGING AND SHELF LIFE; v. 34, p. 11-pg., 2022-11-14.

Abstract

Chitosan and gelatin-based materials containing pomegranate peel extract were prepared for potential application as food packaging materials. An optimized composition of 0.8% chitosan, 0.2% gelatin and 1 mg g-1 of extract was chosen with the aid of a central composite design, presenting low viscosity (8.36 +/- 0.20 Pa s) and solubility (26.00 +/- 5.50%), as well as an expressive inhibition halo against Botrytis cinerea (6.17 +/- 0.36 mm). The interaction between the polymeric matrix and the extract was confirmed by spectroscopic and morphological analysis. Rheologically, the optimized formulation was more resistant to deformation and shear (activation energy increased by almost 5 kJ mol-1) than the formulation without the extract. The use of pomegranate peel extract changed the film color, reduced its solubility (by more than 5%), increased mechanical resistance (tensile strength increased by 15 mPa), and improved light barrier properties by 30%. The extract also imparted antioxidant activity to the film, which was able to inhibit 20% of DPPH (2,2-diphenyl-1-picrylhydrazyl) radical at 38 mg mL-1. The optimized composition also showed inhibitory bacterial properties (31.2 and 125 mu g mL-1) against S. aureus and S. enteritidis, respectively. Thus, the optimized film exhibited interesting characteristics for food packaging applications, many of them brought by the use of pomegranate peel extract, which highlights the valuable contribution of this agro-industrial waste to the properties of the polymeric films. (AU)

• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 14/50857-8 - National Institute in Basic Optics and Applied to Life Sciences
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/13569-8 - Study of the Mechanisms of Action in Photodynamic Therapy: From Photosensitizer to Practical Application
• Grantee: Lucas Danilo Dias
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/24612-9 - Application of nanoemulsions with essential oils as natural coatings for application in post-harvesting strawberry conservation
• Grantee: Josemar Gonçalves de Oliveira Filho
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/22799-7 - Exploring the potential of biopolymers with fruit extracts nanoparticles on controlling food pathogens
• Grantee: Crisiane Aparecida Marangon
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/18748-8 - Chemical composition of strawberries coated with biofilm-based on pomegranate peel extract, chitosan, and gelatin
• Grantee: Mirella Romanelli Vicente Bertolo
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)