Phospholipase cocktail: A new degumming technique for crude soybean oil

Author(s)

dos Passos, Rafaela Menezes ; da Silva, Rubia Mariana ; Pontes, Paula Virginia de Almeida ; Morgano, Marcelo Antonio ; Meirelles, Antonio J. A. ; Stevens, Christian V. ; Ferreira, Marcela Cravo ; Sampaio, Klicia Araujo

• Document type: Journal article
• Source: LWT-FOOD SCIENCE AND TECHNOLOGY; v. 159, p. 9-pg., 2022-02-11.

Abstract

Enzymatic degumming (EDG) is an emerging alternative process for decreasing the phosphorus content, increasing the oil yield, and preserving the oil quality. Purifine (R) 3G is a cocktail of phospholipases composed of phospholipase A2 (PLA2), phospholipase C (PLC), and phosphatidylinositol phospholipase C (PI-PLC). In this study, Purifine (R) 3G was applied to crude soybean oil, and the optimum degumming conditions (enzyme con-centration, temperature, and water dosage) were determined using a central composite rotatable design (CCRD). The contents of diacylglycerols (DAGs) and free fatty acids (FFAs) in the studied system considerably increased at temperatures below 64 C and enzyme concentrations above 100 mg/kg, while the phosphorus content decreased with increasing water amount and enzyme concentration. In particular, EDG with 200 mg/kg of Purifine (R) 3G conducted for 120 min at a temperature of 60 C and water concentration of 3% (w/w) lowered the residual phosphorus content to 8.9 mg/kg and increased the FFA and DAG concentrations by 0.17% and 0.72%, respectively. Meanwhile, EDG retained the tocopherol content in crude soybean oil, maintaining its quality. Hence, Purifine (R) 3G increases the neutral oil yield (FFA and DAG), decreases the phosphorus content, and preserves the oil quality, which make it a commercially viable degumming agent. (AU)

• FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
• Grantee: Roberto de Campos Giordano
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 14/21252-0 - Equilibrium and production processes of biofuels and bioproducts
• Grantee: Antonio José de Almeida Meirelles
• Support Opportunities: Research Projects - Thematic Grants