Synthesis and Characterization of Dendronized Gold Nanoparticles Bearing Charged Peripheral Groups with Antimicrobial Potential

Author(s)

Perli, Gabriel ; Bertuzzi, Diego L. ; Souto, Denio E. P. ; Ramos, Miguel D. ; Braga, Carolyne B. ; Aguiar, Samile B. ; Ornelas, Catia

• Document type: Journal article
• Source: NANOMATERIALS; v. 12, n. 15, p. 15-pg., 2022-08-01.

Abstract

Dendronized gold nanoparticles (AuNPs) were synthesized bearing charged peripheral groups. Two novel AB(3)-type dendrons were synthesized with a thiol group at the focal point followed by their attachment to AuNPs. Dendrons were designed to have nine charged peripheral groups (carboxyl or amine), glycol solubilizing, units and one thiol moiety at the focal point. Both dendrons and all intermediates were synthesized in high yields and characterized by nuclear magnetic resonance spectroscopy (NMR) and mass spectrometry (MS). The amine- and carboxyl-terminated dendrons were used to functionalize gold nanoparticles (AuNPs) previously stabilized with citrate. The nanoparticles' diameters and their colloidal stability were investigated using dynamic light scattering (DLS). The size and morphology of the dendronized AuNPs were evaluated by scanning electron microscopy (SEM), which revealed individual particles with no aggregation after replacement of citrate by the dendrons, in agreement with the DLS data. The absorption spectroscopy reveals a prominent plasmonic band at 560 nm for all AuNPs. The zeta potential further confirmed the expected charged structures of the dendronized AuNPs. Considering all the physical-chemical properties of the charged dendronized AuNPs developed in this work, these AuNPs might be used as a weapon against multi-drug resistant bacterial infections. (AU)

• FAPESP's process: 18/02093-0 - Development of new nanomaterials for nanomedicine applications
• Grantee: Cátia Cristina Capêlo Ornelas Megiatto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/06146-8 - Development of nanocarriers based on dendrimers and polymer nanoparticles for selective delivery of goniothalamin, piplartine and Monastrol
• Grantee: Carolyne Brustolin Braga
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/04929-0 - Development of biosensors for instantaneous detection of proteins and cancer cells, for application on on-site biopsy
• Grantee: Diego Luan Bertuzzi
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 21/00555-9 - Development of polymeric nanoparticles and multifunctional dendrimers for drug delivery
• Grantee: Igor Dias Jurberg
• Support Opportunities: Regular Research Grants