Can the Brazilian Caffeine Expectancy Questionnaires Differentiate the CYP1A2 and ADORA2A Gene Polymorphisms?-An Exploratory Study with Brazilian Athletes

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Mendes, Guilherme Falcao ; Reis, Caio Eduardo Goncalves ; Nakano, Eduardo Yoshio ; Spineli, Higor ; Barreto, Gabriel ; Noronha, Natalia Yumi ; Watanabe, Ligia Moriguchi ; Nonino, Carla Barbosa ; de Araujo, Gustavo Gomes ; Saunders, Bryan ; Zandonadi, Renata Puppin

• Document type: Journal article
• Source: NUTRIENTS; v. 14, n. 16, p. 12-pg., 2022-08-01.

Abstract

This study investigated the ability of the Brazilian Caffeine Expectancy Questionnaire (CaffEQ-BR), full and brief versions, to differentiate genetic profiles regarding the polymorphisms of the CYP1A2 (rs 762551) and ADORA2A (rs 5751876) genes in a cohort of Brazilian athletes. One-hundred and fifty participants were genotyped for CYP1A2 and ADORA2A. After the recruitment and selection phase, 71 (90% male and 10% female, regular caffeine consumers) completed the CaffEQ-BR questionnaires and a self-report online questionnaire concerning sociodemographic data, general health status, and frequency of caffeine consumption. The order of completion of the CaffEQ-BR questionnaires was counterbalanced. The concordance between the full and brief versions of the CaffEQ-BR was analyzed using the intraclass correlation coefficient (ICC). To determine the discriminatory capacity of the questionnaires for genotype, the receiver operating characteristic (ROC) curve was applied for sensitivity and specificity (significance level of 5%). Mean caffeine intake was 244 +/- 161 mg center dot day(-1). The frequency of AA genotypes for CYP1A2 was 47.9% (n = 34) and 52.1% (n = 37) for C-allele carriers (AC and CC). The frequencies of TT genotypes for ADORA2A were 22.7% (n = 15) and 77.3% (n = 51) for C-allele carriers (TC and CC). All CaffEQ-BR factors, for the full and brief versions, were ICCs > 0.75, except for factor 6 (anxiety/negative effects; ICC = 0.60), and presented ROC curve values from 0.464 to 0.624 and 0.443 to 0.575 for CYP1A2 and ADORA2A. Overall, the CaffEQ-BR (full and brief versions) did not show discriminatory capacity for CYP1A2 and ADORA2A gene polymorphisms. In conclusion, the CaffEQ-BR was not able to differentiate genotypes for the CYP1A2 or ADORA2A genes in this group of Brazilian athletes. (AU)

• FAPESP's process: 18/24069-3 - ReSEARCH: Recognizing Signatures of the Exposome to Anticipate the Risks for a Continuous Health
• Grantee: Fernando Barbosa Júnior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 20/12036-3 - The influence of the CYP1A2 polymorphism on the physiological responses and performance following acute supplementation with caffeine
• Grantee: Gabriel Henrique Castanho Barreto
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 21/06836-0 - Placebo in sports and exercise: prevalence, conditioning and open-placebo
• Grantee: Bryan Saunders
• Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
• FAPESP's process: 16/50438-0 - Nutritional suplementation and exercise to optimize exercise performance: focus on individual responses and a step towards personalized sports nutrition
• Grantee: Bryan Saunders
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 20/08687-9 - Selenium deficiency: identification of a risk profile considering genetic, clinical and environmental factors of patients with obesity in the pre and postoperative period of bariatric surgery
• Grantee: Lígia Moriguchi Watanabe
• Support Opportunities: Scholarships in Brazil - Post-Doctoral