Monolayer nanoarchitectonics at the air-water interface for molecular understanding of the interaction of isolinderanolide E with cholesterol

Author(s)

Rosa, Matheus Elias ; Alves Conserva, Geanne A. ; Lago, Joao Henrique G. ; Caseli, Luciano

• Document type: Journal article
• Source: Thin Solid Films; v. 754, p. 7-pg., 2022-07-31.

Abstract

Isolinderanolide E, an alkylated butanolide isolated from Nectandra oppositifolia (Lauraceae), was incorporated in cholesterol Langmuir monolayers. The main insights obtained with the in situ characterizations was that isolinderanolide E fluidized the monolayers, which could not attain rigid states with compression and also decreased the trans/gauche ratio conformers, suggesting molecular disorder. Morphology analysis revealed the formation of typical aggregates upon drug incorporation. These data could be associated with the fact that the monolayers became unstable with isolinderanolide E incorporation, with pre-compressed films decreasing the surface pressure spontaneously with a higher rate than pure cholesterol monolayers. In conclusion, natural isolinderanolide E changed the thermodynamic, mechanical, structural, and morphological cholesterol monolayers, which may be essential to understand the action at the molecular level bioactives in selected membrane models. (AU)

• FAPESP's process: 19/03239-0 - Nanostructured interfaces for the investigation of bioactive substances in cell membrane models and for the construction of optoelectronic devices
• Grantee: Luciano Caseli
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/20633-6 - Bioactive metabolites from Nectandra oppositifolia Nees & Mart. (Lauraceae): molecular characterization, in vitro and in vivo antiparasitic potential evaluation and determination of mechanism of action
• Grantee: Geanne Alexsandra Alves Conserva
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 21/02789-7 - Search for bioactive metabolites with antiparasitic action in plant species from Atlantic Forest and Cerrado regions - a chemical, phenotypical, and metabolomic approach
• Grantee: João Henrique Ghilardi Lago
• Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants
• FAPESP's process: 19/04407-4 - Study of the surface activity of alkylated lactones and their interaction in cell membrane models
• Grantee: Matheus Elias Rosa
• Support Opportunities: Scholarships in Brazil - Scientific Initiation