Dynamic gastrointestinal digestion/intestinal permeability of encapsulated and nonencapsulated Brazilian red propolis: Active compounds stability and bioactivity

Author(s)

de Alencar, Severino Matias ; Sartori, Alan Giovanini de Oliveira ; Dag, Damla ; Batista, Pollyanna Souza ; Rosalen, Pedro Luiz ; Ikegaki, Masaharu ; Kong, Fanbin

• Document type: Journal article
• Source: Food Chemistry; v. 411, p. 9-pg., 2023-01-19.

Abstract

The objectives were to investigate the effect of dynamic gastrointestinal digestion/Caco-2 cell transport on active compounds stability and antioxidant/anti-inflammatory activities of the ethanolic extract of Brazilian red propolis (EEBRP), whether encapsulated or not; and the in vivo acute toxicity of the EEBRP after digestion. Eight isoflavonoids, one flavanone, and one chalcone were identified by HPLC-ESI-QTOF-MS, and quantified by HPLCPDA. Bioaccessibility was higher for the encapsulated EEBRP (21.4%-57.6%) than for the nonencapsulated (19.3%-30.2%). Conversely, the Caco-2 cell transport was higher for the nonencapsulated EEBRP. Similarly, the nonencapsulated EEBRP showed higher ability to scavenge reactive oxygen species, which was especially attributed to calycosin, and to decrease NF-kappa B activation, and the levels of TNF-alpha and CXCL2/MIP-2 after Caco-2 cell transport. Hence, there is an indication that EEBRP is a promising alternative dietary source of bioavailable isoflavonoids. Further studies on encapsulation should be encouraged to improve bioactivity, and expand its food applications. (AU)

• FAPESP's process: 19/11248-0 - Production of Brazilian red propolis microcapsules for use as a natural preservative and development of functional foods: characterization, bioaccessibility and epithelial transport through Caco-2 cells
• Grantee: Severino Matias de Alencar
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/17426-7 - Change of isoflavonoid pattern in Brazilian red propolis after dynamic gastrointestinal digestion and transport by Caco-2 cells
• Grantee: Severino Matias de Alencar
• Support Opportunities: Scholarships abroad - Research