DNA methylation analysis in transcriptional regulation comprehension of relevant g...
Comparative genome hybridization (CGH) and epigenetic regulation in neoplasms, in ...
| Full text | |
| Author(s): Show less - |
Wisnieski, Fernanda
;
Geraldis, Jaqueline Cruz
;
Santos, Leonardo Caires
;
Leal, Mariana Ferreira
;
Calcagno, Danielle Queiroz
;
Gigek, Carolina Oliveira
;
Chen, Elizabeth Suchi
;
Anauate, Ana Carolina
;
Artigiani, Ricardo
;
Demachki, Samia
;
Assumpcao, Paulo Pimentel
;
Lourenco, Laercio Gomes
;
Arasaki, Carlos Haruo
;
Krainer, Julie
;
Pabinger, Stephan
;
Burbano, Rommel Rodriguez
;
Cardoso Smith, Marilia Arruda
Total Authors: 17
|
| Document type: | Journal article |
| Source: | Epigenetics; v. 17, n. 1, p. 7-pg., 2021-02-07. |
| Abstract | |
Gastric cancer (GC) is one of the leading types of fatal cancer worldwide. Epigenetic manipulation of cancer cells is a useful tool to better understand gene expression regulatory mechanisms and contributes to the discovery of novel biomarkers. Our research group recently reported a list of 83 genes that are potentially modulated by DNA methylation in GC cell lines. Herein, we further explored the regulation of one of these genes, LRRC37A2, in clinical samples. LRRC37A2 expression was evaluated by RT-qPCR, and DNA methylation was studied using next-generation bisulphite sequencing in 36 GC and paired adjacent nonneoplastic tissue samples. We showed that both reduced LRRC37A2 mRNA levels and increased LRRC37A2 exon methylation were associated with undifferentiated and poorly differentiated tumours. Moreover, LRRC37A2 gene expression and methylation levels were inversely correlated at the +45 exon CpG site. We suggest that DNA hypermethylation may contribute to reducing LRRC37A2 expression in undifferentiated and poorly differentiated GC. Therefore, our results show how some genes may be useful to stratify patients who are more likely to benefit from epigenetic therapy. (AU) | |
| FAPESP's process: | 07/02470-3 - Differential phospho and glyco proteomic analysis in gastric adenocarcinoma and the eventual correlation with clinic-pathological parameters of prognosis |
| Grantee: | Mariana Ferreira Leal |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 17/06227-8 - DNA methylation analysis in transcriptional regulation comprehension of relevant genes in gastric carcinogesis |
| Grantee: | Jaqueline Cruz Geraldis |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 09/07145-9 - Genetic and epigenetic aspects in gastric carcinogenesis |
| Grantee: | Marilia de Arruda Cardoso Smith |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 10/11174-1 - Genetic and epigenetic evaluation in gastric cancer |
| Grantee: | Danielle Queiroz Calcagno |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 16/19953-6 - Epigenetically modulated genes may constitute therapeutic targets in patients with gastric cancer? |
| Grantee: | Fernanda Wisnieski |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |