Sonophotodynamic inactivation of Pseudomonas aeruginosa biofilm mediated by curcumin

Author(s)

Silva e Carvalho, Iago ; Pratavieira, Sebastiao ; Bagnato, Vanderlei Salvador ; Alves, Fernanda

• Document type: Journal article
• Source: BIOFOULING; v. 39, n. 6, p. 11-pg., 2023-07-26.

Abstract

The inactivation of Pseudomonas aeruginosa biofilm is a major challenge, as biofilms are less responsive to conventional treatments and responsible for persistent infections. This has led to the investigation of alternative approaches for biofilm control such as photodynamic (PDI) and sonodynamic (SDI) inactivation. The combination of them, known as Sonophotodynamic Inactivation (SPDI), has improved the effectiveness of the process. Curcumin, a well-established photosensitizer, has been identified as a potential sonosensitizer. This study evaluated the most effective combination for SPDI against P. aeruginosa biofilms in vitro, varying curcumin concentrations and ultrasound intensities. The results indicated that the inactivation was directly proportional to the curcumin concentration. Using curcumin 120 & mu;M and 3.0 W.cm(-2) of ultrasound intensity, SPDI demonstrated the highest and the best synergistic results, equivalent to 6.9 & PLUSMN; 2.1 logs of reduction. PDI reduced 0.7 & PLUSMN; 0.9 log and SDI had no effect. In conclusion, SPDI with curcumin is a promising approach for biofilm inactivation. (AU)

• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 21/01324-0 - Extracellular matrix disruption and the use of a curcumin nanoparticle to potentiate the inactivation of Staphylococcus aureus biofilms
• Grantee: Fernanda Alves Dias de Sousa
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 14/50857-8 - National Institute in Basic Optics and Applied to Life Sciences
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Projects - Thematic Grants