3D-printed amperometric sensor for the detection of ethanol in saliva

Author(s)

von Zuben, Theodora Wrobel ; Kalinke, Cristiane ; Janegitz, Bruno Campos ; Salles Jr, Airton Goncalves ; Bonacin, Juliano Alves

• Document type: Journal article
• Source: Electroanalysis; v. N/A, p. 11-pg., 2023-09-29.

Abstract

In this work, we report a non-enzymatic and metal-free ethanol sensor based on 3D PLA-graphene electrodes. We evaluated the optimal activation treatment for PLA-graphene to achieve a sensor for monitoring ethanol levels in saliva samples, following the legal limits for drivers in various countries. The analytical performance of the sensor was determined through amperometric measurements, demonstrating a linear detection ranging supporting electrolyte (0.990-19.3 mmol L-1) and artificial saliva sample (0.990-17.4 mmol L-1), with respective limits of detection of 0.135 and 0.239 mmol L-1. The 3D-printed sensor exhibited excellent repeatability and reproducibility. This method was effectively employed for ethanol detection, highlighting its potential as an alternative approach for assessing ethanol levels in drivers while considering diverse legal regulations across different countries. image (AU)

• FAPESP's process: 21/07989-4 - Additive-manufactured (3D printed) electrochemical devices for the diagnosis of viral diseases
• Grantee: Cristiane Kalinke
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 19/21506-6 - Development of new synthetic methods in line with the green chemistry targeting complex molecules: multicomponent benzannulation, visible-light driven synthesis of dienes in presence of copper and preparation of bicyclic furan/8-membered ring ketones
• Grantee: Airton Goncalves Salles Junior
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/22127-2 - Development of novel materials strategic for integrated analytical devices
• Grantee: Lauro Tatsuo Kubota
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/00473-2 - Development of 3-dimensional (3D) printed electrochemical biosensors with PLA polymer and graphene for the determination of biomolecules and diagnosis of diseases
• Grantee: Cristiane Kalinke
• Support Opportunities: Scholarships in Brazil - Post-Doctoral