A New Molecularly Imprinted Polymer for In-Tube SPME/UHPLC-MS/MS of Anandamide in Plasma Samples

Author(s)

Pinto, Mania A. L. ; de Souza, Israel D. ; Miranda, Luis Felippe C. ; Queiroz, Maria Eugenia C.

• Document type: Journal article
• Source: Journal of the Brazilian Chemical Society; v. N/A, p. 9-pg., 2023-05-16.

Abstract

Parkinson's disease is a neurodegenerative disorder characterized by progressive loss of dopaminergic neurons. There is substantial evidence that the endocannabinoid system modulates the dopaminergic activity in the basal ganglia, a forebrain system that integrates cortical information to coordinate motor activity regulating signals. In this article, a fused-silica capillary with a molecularly imprinted polymer was developed for in-tube solid-phase microextraction of the endocannabinoid anandamide in plasma samples from Parkinson's disease patients and further analysis by ultra-high-performance liquid chromatography with tandem mass spectrometry. The molecularly imprinted polymer capillary presented recognition sites with complementary shape, size, and functionality to anandamide. Scanning electron micrographs and Fouriertransform infrared spectra illustrated the physical and chemical modification of the printed and non-printed capillary surface. The in-tube solid-phase microextraction ultra-high-performance liquid chromatography with tandem mass spectrometry method presented a linear range from 0.1 to 20 ng mL-1, precision with coefficient of variation values ranging from 1.2 to 13%, and relative standard deviation accuracy ranging from -3.6 to 7.5%. The method developed herein can adequately determine anandamide in plasma samples from Parkinson's disease patients. By applying the standard addition approach, the anandamide plasmatic concentration in these samples was found to range from 0.2 to 0.4 ng mL-1. (AU)

• FAPESP's process: 13/03808-9 - Development of extraction phase material restricted to microextraction packed sorbent drug in plasma samples and analysis by liquid chromatography coupled to mass spectrometry sequence
• Grantee: Mônia Aparecida Lemos Pinto de Melo
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/07619-1 - Column switching liquid chromatography coupled to tandem mass spectrometry for the analysis of endocannabinoids and drugs in biological samples
• Grantee: Maria Eugênia Queiroz Nassur
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications
• Grantee: Fernando Mauro Lanças
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 20/00126-8 - Development of the methods of the microextraction hyphenated with LC-MS/MS or directly to MS/MS for the determination of biomarkers in biological samples of patient or rats with neurodegenerative diseases
• Grantee: Maria Eugênia Queiroz Nassur
• Support Opportunities: Regular Research Grants
• FAPESP's process: 19/19485-0 - Development of nitinol fiber chemically coated with zwitterionic polymeric ionic liquids for determination of beta-amyloids in plasma samples from patients with Alzheimer's disease by direct coupling fiber-in-tube SPME and MS/MS
• Grantee: Israel Donizeti de Souza
• Support Opportunities: Scholarships in Brazil - Post-Doctoral