Effects of probenecid and brilliant blue G on rat enteric glial cells following intestinal ischemia and reperfusion

Author(s)

Mendes, Cristina Eusebio ; Palombit, Kelly ; Pereira, Thaira Thalita Alves ; Magalhaes, Henrique Inhauser Riceti ; Caetano, Marcos Antontio Ferreira ; Castelucci, Patricia

• Document type: Journal article
• Source: ACTA HISTOCHEMICA; v. 125, n. 1, p. 13-pg., 2022-12-07.

Abstract

The P2X7 receptor participates in several intracellular events and acts with the pannexin-1 channel. This study examined the effects of probenecid (PB) and brilliant blue G (BBG), which are antagonists of the pannexin-1 channel and P2X7 receptor, respectively, on rat ileum enteric glial cells after on ischemia and reperfusion. The ileal vessels were occluded for 45 min with nontraumatic vascular tweezers, and reperfusion was performed for periods of 24 h and 14 and 28 days. After ischemia (IR groups), the animals were treated with BBG (BG group) or PB (PB group). The double-labeling results demonstrated the following: the P2X7 receptor was present in enteric glial cells (S100 beta) and enteric neurons positive for HuC/D; enteric glial cells exhibited different phenotypes; some enteric glial cells were immunoreactive to only S100 beta or GFAP; and the pannexin-1 channel was present in enteric glial cells (GFAP). Density (in cells/cm2) analyses showed that the IR group exhibited a decrease in the number of cells immunoreactive for the P2X7 receptor, pannexin-1, and HuC/D and that treatment with BBG or PB resulted in the recovery of the numbers of these cells. The number of glial cells (S100 beta and GFAP) was higher in the IR group, and the treatments decreased the number of these cells to the normal value. However, the PB group did not exhibit recovery of S100 beta-positive glia. The cell profile area (mu m2) of S100 beta-positive enteric glial cells decreased to the normal value after BBG treatment, whereas no recovery was observed in the PB group. The ileum contractile activity was decreased in the IR group and returned to baseline in the BG and PB groups. BBG and PB can effectively induce the recovery of neurons and glia cells and are thus potential therapeutic agents in the treatment of gastrointestinal tract diseases. (AU)

• FAPESP's process: 15/22299-3 - Study of the pannexin-1 channel in the myenteric plexus following ischemia and reperfusion
• Grantee: Thaira Thalita Alves Pereira
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/00259-1 - Role of the brilliant blue green (BBG) antagonist on the P2X7 receptor and enteric nervous system of the ileum rats submitted to ischemia and reperfusion: morphofunctional study
• Grantee: Patricia Castelucci
• Support Opportunities: Regular Research Grants
• FAPESP's process: 18/07862-1 - Study of the effect of experimental ulcerative colitis on the enteric nervous system of P2X7 receptor deficient mice (P2X7-/-)
• Grantee: Patricia Castelucci
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/25927-2 - Morphological, molecular and functional aspects of the interaction between the P2X7 receptor and pannexin-1 in the enteric glial cells following intestinal ischemia/reperfusion
• Grantee: Patricia Castelucci
• Support Opportunities: Regular Research Grants