Titanium biogenic nanoparticles to help the growth of Trichoderma harzianum to be used in biological control

Author(s):Show less -

Pasquoto-Stigliani, Tatiane ; Guilger-Casagrande, Mariana ; Campos, Estefania V. R. ; Germano-Costa, Tais ; Bilesky-Jose, Natalia ; Migliorini, Bianca B. ; Feitosa, Leandro O. ; Sousa, Bruno T. ; de Oliveira, Halley C. ; Fraceto, Leonardo F. ; Lima, Renata

• Document type: Journal article
• Source: JOURNAL OF NANOBIOTECHNOLOGY; v. 21, n. 1, p. 21-pg., 2023-05-25.

Abstract

Background The biogenic synthesis of metallic nanoparticles is a green alternative that reduces the toxicity of this nanomaterials and may enable a synergy between the metallic core and the biomolecules employed in the process enhancing biological activity. The aim of this study was to synthesize biogenic titanium nanoparticles using the filtrate of the fungus Trichoderma harzianum as a stabilizing agent, to obtain a potential biological activity against phytopathogens and mainly stimulate the growth of T. harzianum, enhancing its efficacy for biological control.Results The synthesis was successful and reproductive structures remained in the suspension, showing faster and larger mycelial growth compared to commercial T. harzianum and filtrate. The nanoparticles with residual T. harzianum growth showed inhibitory potential against Sclerotinia sclerotiorum mycelial growth and the formation of new resistant structures. A great chitinolytic activity of the nanoparticles was observed in comparison with T. harzianum. In regard to toxicity evaluation, an absence of cytotoxicity and a protective effect of the nanoparticles was observed through MTT and Trypan blue assay. No genotoxicity was observed on V79-4 and 3T3 cell lines while HaCat showed higher sensitivity. Microorganisms of agricultural importance were not affected by the exposure to the nanoparticles, however a decrease in the number of nitrogen cycling bacteria was observed. In regard to phytotoxicity, the nanoparticles did not cause morphological and biochemical changes on soybean plants.Conclusion The production of biogenic nanoparticles was an essential factor in stimulating or maintaining structures that are important for biological control, showing that this may be an essential strategy to stimulate the growth of biocontrol organisms to promote more sustainable agriculture. (AU)

• FAPESP's process: 16/13692-6 - Silver biogenic nanoparticles based on Trichoderma harzianum: different synthesis conditions, evaluation of cyto/genotoxicity and inhibitory potential of Sclerotinia sclerotiorum
• Grantee: Mariana Guilger Casagrande
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 20/05816-2 - Study of the capping of biogenic nanoparticles and their correlation with toxicity and activity in the control of phytopathogens
• Grantee: Mariana Guilger Casagrande
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/13328-5 - Development and evaluation of biogenic metal nanoparticles (silver, titanium and iron) based on Trichoderma harzianum for agricultural application
• Grantee: Renata de Lima
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/21004-5 - Agriculture, micro/nanotechnology and environment: from evaluation of the mechanisms of action to studies of transport and toxicity
• Grantee: Leonardo Fernandes Fraceto
• Support Opportunities: Research Projects - Thematic Grants