Macrophages CD163+and Factor XIIIa plus Provide a First-Line Defence against Proliferative Verrucous Leukoplakia Antigens

Author(s)

Palacon, Mariana Paravani ; Barbeiro, Camila de Oliveira ; Fernandes, Darcy ; Biancardi, Mariel Ruivo ; Silveira, Heitor Albergoni ; Ferrisse, Tulio Morandin ; Leon, Jorge Esquiche ; Kujan, Omar ; Bufalino, Andreia

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 24, n. 6, p. 12-pg., 2023-03-01.

Abstract

This study aimed to evaluate the density of the dendritic cells (DCs) and macrophages in oral leukoplakia (OL) and proliferative verrucous leukoplakia (PVL) by immunohistochemical analysis. We analysed paraffined tissue samples of PVL (n = 27), OL (n = 20), and inflammatory fibrous hyperplasia (n = 20) as the control group using the immunomarkers for DCs (CD1a, CD207, CD83, CD208 and CD123) and macrophages (CD68, CD163, FXIIIa and CD209). A quantitative analysis of positive cells in the epithelial and subepithelial areas was determined. Our results showed a reduction in CD208+ cells in the subepithelial area of the OL and PVL compared to the control. Additionally, we found a higher density of FXIIIa+ and CD163+ cells in the subepithelial area in PVL compared to the OL and control. Four-way MANOVA revealed a relationship between increased CD123+ cell density in the subepithelial area of "high-risk" samples regardless of disease. Macrophages provide the first line of defence against PVL antigens, suggesting a distinct pattern of innate immune system activation in PVL compared to OL, which may contribute to the complexity and the high rate of malignant transformation in the PVL. (AU)

• FAPESP's process: 18/04954-2 - Evaluation of the acetylation status of H3 histone and its contribution to genomic instability in oral leukoplakia and Proliferative Verrucous Leukoplakia
• Grantee: Camila de Oliveira Barbeiro
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 18/22236-0 - Comparative analysis of lymphocytic infiltrate in oral leucoplastic lesions and oral lichen planus
• Grantee: Mariana Paravani Palaçon
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 13/07276-1 - CEPOF - Optics and Photonic Research Center
• Grantee: Vanderlei Salvador Bagnato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 21/01191-0 - Tolerance induction and Candida albicans's biofilm characterization after antimicrobial photodymamic therapy application
• Grantee: Túlio Morandin Ferrisse
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/01798-7 - In vitro study of the modulation of innate and adaptive immunity by dysplastic keratinocytes
• Grantee: Darcy Fernandes
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/17288-8 - Comparative immunohistochemical characterization of subgroups of dendritic cells, macrophages and cytotoxic T lymphocytes in oral leukoplakia and proliferative verrucous leukoplakia
• Grantee: Mariel Ruivo Biancardi
• Support Opportunities: Scholarships in Brazil - Scientific Initiation