Diversity of bacterial and fungal endophytic communities presents in the leaf blades of Sinningia magnifica, Sinningia schiffneri and Sinningia speciosa from different cladus of Gesneriaceae family: A comparative analysis in three consecutive years

Author(s)

Hernandez-Tasco, Alvaro Jose ; Tronchini, Rafaela Aparecida ; Apaza-Castillo, Gladys Angelica ; Hosaka, Guilherme Kenichi ; Quinones, Nataly Ruiz ; Goulart, Marcela Cristina ; Fantinatti-Garboggini, Fabiana ; Salvador, Marcos Jose

• Document type: Journal article
• Source: MICROBIOLOGICAL RESEARCH; v. 271, p. 10-pg., 2023-04-01.

Abstract

Sinningia is a genus of plants of Gesneriaceae family with species native to Brazil and is a source of several classes of bioactive secondary metabolites, such as quinones, terpenoids, flavonoids, and phenylethanoid glycosides. However, the diversity of endophytic microorganisms associated with them and the impact of endophytes on the biosynthesis of bioactive substances is unknown. Therefore, we sought to evaluate the microbial diversity, behavior, and frequency of endophytes in leaves blades of S. magnifica, S. schiffneri, and S. speciosa. These plants were collected in different regions and environments of Brazil and were studied comparatively for three consecutive years. The total DNA obtained from the blades of the plant leaves were sequenced by the Illumina MiSeq platform, followed by bioinformatics analysis to assess the microbial diversity of endophytes associated with each plant species and study year. The results of the taxonomic diversity showed a dynamic microbial community, which contained several bacterial phyla among them, Actinomycetota, Bacteroidota, Bacillota, and Pseudomonadota, and for the fungal phyla Ascomycota and Basidiomycota. Comparing the three years of study, the richness of the genera, over time, was decreasing, with signs of recovery towards the third year. The alpha and beta diversity indices confirm a great phylogenetic richness in the endophytic communities of bacteria and fungi associated with the leaf blades of Sinningia. However, these communities are comparatively little conserved, showing population and taxonomic changes of the microorganisms over time, possibly as a measure of adjustment to environmental conditions, evidencing both its fragility and versatility against the effects of environmental change on its endophytic microbial communities. (AU)

• FAPESP's process: 21/09693-5 - Use of microbiome technologies and bioassays for the search for new bioactive compounds in plants and symbionts: implications for metabolic responses, molecular diversity and bioactivity
• Grantee: Marcos José Salvador
• Support Opportunities: BIOTA-FAPESP Program - Regular Research Grants
• FAPESP's process: 15/03726-8 - Integrated chemical and biological study in the search for bioactive substances in plants* and development of analytical procedures. *(Amaranthaceae, Gesneriaceae, Myrtaceae e Annonaceae)
• Grantee: Marcos José Salvador
• Support Opportunities: Regular Research Grants
• FAPESP's process: 17/03598-5 - Preventive effect of Ilex paraguariensis preparations (chimarrão, terere and mate tea) against damages caused by chronic ethanol consumption: comparative analysis of preparation technology, chemical profile and evaluation of therapeutic effect and safety
• Grantee: Mariana Mesquita Fonseca
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 06/06079-4 - Search of antioxidants and antimicrobials in Gomphreneae tribe species
• Grantee: Marcos José Salvador
• Support Opportunities: Regular Research Grants
• FAPESP's process: 12/09101-1 - Investigation of treatment with 24-epibrassinolide in plant development, profile of volatile constituents and biological activities of essential oils from Cymbopogon citratus, Cymbopogon flexuosus and Mentha pulegium
• Grantee: Marcos José Salvador
• Support Opportunities: Regular Research Grants