Analysis Delta 9-THC in cosmetics by high performance liquid chromatography with UV-Vis detection

Author(s)

Teles de Menezes, Matheus Manoel ; de Andrade, Jose Fernando ; de Oliveira, Marcelo Firmino ; Tristao, Heloisa Maria ; Saczk, Adelir Aparecida ; Okumura, Leonardo Luiz

• Document type: Journal article
• Source: BRAZILIAN JOURNAL OF ANALYTICAL CHEMISTRY; v. 2, n. 8, p. 4-pg., 2012-04-01.

Abstract

A chromatographic methodology for Delta 9-THC analysis in cosmetics is described. The HPLCUV-Vis technique was employed for quantification of Delta 9-THC in the 1.0 to 100 ppm working range using an optimized mobile phase consisting of methanol/water (4:1). In these experimental conditions, Delta 9-THC presented a retention time of 23.7 minutes at the optimized wavelength of 209 nm. The analytical curve presented a linear correlation coefficient of 3.136x10(-6) and the limits of detection and quantification were 0.746 and 2.487 ppm, respectively. This methodology was employed to quantify Delta 9-THC in hemp oil-based cosmetics and it was possible to detect this cannabinol in concentrations ranging from 0.728 to 2.672 ppm. (AU)

• FAPESP's process: 12/00084-7 - Development of chemically modified electrodes for the voltammetric determination of cocaine, MDMA, LSD, and D-9-tetrahydrocannabinol in samples of forensic interest
• Grantee: Marcelo Firmino de Oliveira
• Support Opportunities: Regular Research Grants