Modulatory Effect of Betulinic Acid on the Genotoxicity Induced by Different Mutagens in V79 Cells

Author(s)

Acesio, Nathalia Oliveira ; de Oliveira, Pollyanna Francielli ; Pereira Mastrocola, Daiane Fernanda ; de Souza Lima, Ildercilio Mota ; Munari, Carla Carolina ; Ferreira Lucatti Sato, Vania Luiza ; Silva Souza, Andressa Aparecida ; Bocalon Flauzino, Luzio Gabriel ; Cunha, Wilson Roberto ; Tavares, Denise Crispim

• Document type: Journal article
• Source: Evidence-based Complementary and Alternative Medicine; v. 2016, p. 6-pg., 2016-01-01.

Abstract

Betulinic acid (BA) is a pentacyclic triterpene that can be isolated from many medicinal plants around the world. The aim of this study was to evaluate the genotoxic potential of BA and its effect on the genotoxicity induced by different mutagens in V79 cells using the cytokinesis-block micronucleus assay. Different BA concentrations were combined with methyl methanesulfonate (MMS), doxorubicin (DXR), camptothecin (CPT), and etoposide (VP-16). The frequencies of micronuclei in cultures treated with different BA concentrations did not differ from those of the negative control. Treatment with BA and MMS resulted in lower micronucleus frequencies than those observed for cultures treated with MMS alone. On the other hand, a significant increase in micronucleus frequencies was observed in cultures treated with BA combined with DXR or VP-16 when compared to these mutagens alone. The results showed no effect of BA on CPT-induced genotoxicity. Therefore, BA was not genotoxic under the present experimental conditions and exerted a different influence on the genotoxicity induced by different mutagens. The modulatory effect of BA depends on the type of mutagen and concentrations used. (AU)

• FAPESP's process: 12/16182-8 - Chemical study and biological assays with species of the cerrado searching to antiparasitic substances
• Grantee: Wilson Roberto Cunha
• Support Opportunities: Regular Research Grants
• FAPESP's process: 11/05732-4 - Study of cytotoxic and genotoxic potential of Solanum lycocarpum and their majoritary compounds and their influence on genotoxicity induced by different mutagens
• Grantee: Denise Crispim Tavares
• Support Opportunities: Regular Research Grants