LC-MS/MS methods for sulfadimethoxine and ormetoprim analysis in feed and fish fillet and a leaching study for feed after alternative procedures for the incorporation of drugs

Author(s)

Fais, Ana Paula ; Barboza Franco, Rodolfo Scarpino ; Baldo da Silva, Agnaldo Fernando ; de Freitas, Osvaldo ; Rizzato Paschoal, Jonas Augusto

• Document type: Journal article
• Source: Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure; v. 34, n. 4, p. 8-pg., 2017-01-01.

Abstract

(T)his paper describes the method development for sulfadimethoxine (SDM) and ormetoprim (OMP) quantitation in fish feed and fish fillet employing LC-MS/MS. In order to assess the reliability of the analytical method, valuation was undertaken as recommended by guidelines proposed by the Brazilian Ministry of Agriculture. The calibration curve for the quantification of both drugs in feed showed adequate linearity (r > 0.99), precision (CV < 12%) and trueness ranging from 97% to 100%. The method for the determination of SDM and OMP residues in fish fillet involved a simple sample preparation procedure that had adequate linearity (r > 0.99), precision (CV < 16%) and trueness around 100%, with CCa < 100.2 ng g(-1) and CC beta < 100.4 ng g(-1). With a goal of avoiding the risk of drug leaching from feed into the aquatic environment during fish medication via the oral route, different procedures for drug incorporation into feed were evaluated. Coating feed pellets with ethyl cellulose polymer containing the drug showed promising results. In this case, medicated feed released drugs to water at a level below 6% when the medicated feed stayed in the water for up to 15 min. (AU)

• FAPESP's process: 12/18334-0 - Veterinary drug residues in food: multiclass analytical methods employing LC-MS/MS, exposition data and residual depletion study
• Grantee: Jonas Augusto Rizzato Paschoal
• Support Opportunities: Research Grants - Young Investigators Grants