Veterinary drug residues in food: multiclass analytical methods employing LC-MS/MS, exposition data and residual depletion study

• Grant number: 12/18334-0
• Support Opportunities: Research Grants - Young Investigators Grants
• Field of knowledge: Agronomical Sciences - Food Science and Technology - Food Science
• Principal Investigator: Jonas Augusto Rizzato Paschoal
• Grantee: Jonas Augusto Rizzato Paschoal
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated researchers: José Eurico Possebon Cyrino ; Severino Matias de Alencar ; Valdemar Luiz Tornisielo
• Associated research grant(s): 14/50755-0 - Metaqua: metabolite/metabolomic profiling in aquaculture research with a view to maintaining food safety / security and animal / human health, AP.R SPRINT
• Associated scholarship(s): 18/05536-0 - Erythromycin in aquaculture: studies for drug incorporation in feed and for residual depletion in fish, BP.MS; 17/15052-7 - Residual persistence investigation of toxic constituents of C. duckei used in Pisciculture, BP.PD; 16/16593-9 - Estimation and evaluation of withdrawal periods for albendazole and its metabolites in fish, BP.MS; + associated scholarships 14/06909-3 - Development of analytical and confirmatory methods for multi-class residues of veterinary drugs determination in milk and beef meat using LC-MS/MS, BP.IC; 14/06852-1 - Residue depletion study of veterinary drugs in fish and estimation of grace periods, BP.IC; 13/16351-7 - Veterinary drug residues in food: multiclass analytical methods employing LC-MS/MS, exposition data and residual depletion study, BP.JP - associated scholarships

Abstract

The veterinary pharmaceutical industry market in the world and even in Brazil, has shown significant growth over the last few years, highlighting the importance of using these substances in different animal production systems and advising on the need for inspection of products intended human consumption with regard to the issue of food security. Consistently, researchers have been working to improve the analytical methods in order to obtain reliable results even in trace concentrations of these substances (µg/kg). A major trend in recent analytical methods published in scientific literature search reveals the possibility of determining simultaneously the maximum number of compounds of veterinary drugs as possible from different therapeutic classes (antimicrobial, antiparasitic, etc.) in different food matrices in the same analysis. However, unlike what is already a reality for methods aimed at determination of pesticide residues, there are few multiresidue methods for veterinary drugs from different classes, able to meet the criteria needed for a wide range of different compounds. Brazil has national programs to monitoring of drugs (and possible metabolites) in many different food products of animal origin, but still lacking the availability of analytical methods like multiclass. Against this background, this project is proposed in order to develop and validate analytical methods multiresidues, which can be applied for determining the largest number of veterinary drugs as possible in different classes (multiclass) and with the versatility to be used in analyzes of different food matrices, with the aim of contributing to national programs for monitoring these substances in foodstuffs of animal origin. Based on the methods developed, it is proposed to undertake analyzes of different food matrices, from products available to consumers in the cities of São Paulo, in order to contribute to national regulators, with results that allow an assessment of the panorama exposure of consumers to these compounds. Due to lack of veterinary medicines properly regulated in Brazil to be employed in aquaculture production systems, this project also aims to conduct studies of depletion of residual drugs already regulated for use in fish farming in other countries, with the objective of estimating the period need for major fish species commercially grown in Brazil. (AU)

Scientific publications

(11)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

FAIS, ANA PAULA; BARBOZA FRANCO, RODOLFO SCARPINO; BALDO DA SILVA, AGNALDO FERNANDO; DE FREITAS, OSVALDO; RIZZATO PASCHOAL, JONAS AUGUSTO. LC-MS/MS methods for sulfadimethoxine and ormetoprim analysis in feed and fish fillet and a leaching study for feed after alternative procedures for the incorporation of drugs. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure, v. 34, n. 4, SI, p. 501-508, 2017. (12/18334-0)

VELLOSA PORTELA, ANA CAROLINA; FERNANDES SILVEIRA, JULIANA GRELL; DAMACENO, MARINA ALVES; BALDO DA SILVA, AGNALDO FERNANDO; DE JESUS, RAPHAEL BARBETTA; PILARSKI, FABIANA; GADAJ, ANNA; MOONEY, MARK H.; RIZZATO PASCHOAL, JONAS AUGUSTO. Food safety evaluation for the use of albendazole in fish: residual depletion profile and withdrawal period estimation. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure, v. 37, n. 4, FEB 2020. (12/18334-0, 16/16593-9, 14/50755-0)

DA SILVA, JONAS JOAQUIM MANGABEIRA; CAMPANHARO, SARAH CHAGAS; PASCHOAL, JONAS AUGUSTO RIZZATO. Ethnoveterinary for food-producing animals and related food safety issues: A comprehensive overview about terpenes. COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY, v. 20, n. 1, p. 48-90, JAN 2021. (19/06159-8, 17/15052-7, 18/05536-0, 12/18334-0)

DA SILVA, JONAS JOAQUIM MANGABEIRA; CAMPANHARO, SARAH CHAGAS; DA SILVA, AGNALDO FERNANDO BALDO; DE JESUS, RAPHAEL BARBETTA; FIGUEREDO, TAIS ALEXANDRA MARIO; PILARSKI, FABIANA; HELENO, VLADIMIR CONSTANTINO GOMES; PASCHOAL, JONAS AUGUSTO RIZZATO. Combination of extractive techniques followed by HPLC-MS/MS analysis to monitor ent-agathic acid in fish treated with Copaifera duckei Dwyer. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v. 1224, p. 7-pg., 2023-05-26. (19/06159-8, 17/15052-7, 12/18334-0)

BUSATTO, ZENAIS; BALDO DA SILVA, AGNALDO FERNANDO; DE FREITAS, OSVALDO; RIZZATO PASCHOAL, JONAS AUGUSTO. LC-MS/MS methods for albendazole analysis in feed and its metabolite residues in fish fillet and a leaching study in feed after an alternative procedure for drug incorporation. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure, v. 34, n. 4, p. 11-pg., 2017-01-01. (12/18334-0)

BUSATTO, ZENAIS; BALDO DA SILVA, AGNALDO FERNANDO; DE FREITAS, OSVALDO; RIZZATO PASCHOAL, JONAS AUGUSTO. LC-MS/MS methods for albendazole analysis in feed and its metabolite residues in fish fillet and a leaching study in feed after an alternative procedure for drug incorporation. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure, v. 34, n. 4, SI, p. 509-519, 2017. (12/18334-0)

BUSATTO, ZENAIS; DE FRANCA, WELLITON GONCALVES; POSSEBON CYRINO, JOSE EURICO; RIZZATO PASCHOAL, JONAS AUGUSTO. Assessment of elimination profile of albendazole residues in fish. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure, v. 35, n. 1, p. 77-85, 2018. (12/18334-0)

FAIS, ANA PAULA; BARBOZA FRANCO, RODOLFO SCARPINO; BALDO DA SILVA, AGNALDO FERNANDO; DE FREITAS, OSVALDO; RIZZATO PASCHOAL, JONAS AUGUSTO. LC-MS/MS methods for sulfadimethoxine and ormetoprim analysis in feed and fish fillet and a leaching study for feed after alternative procedures for the incorporation of drugs. Food Additives and Contaminants Part A-Chemistry Analysis Control Exposure, v. 34, n. 4, p. 8-pg., 2017-01-01. (12/18334-0)

DE FREITAS, LUCAS VICTOR PEREIRA; DA MOTA, CARLOS AUGUSTO ALVARENGA; DAMACENO, MARINA ALVES; SILVEIRA, JULIANA GRELL FERNANDES; PORTELA, ANA CAROLINA VELLOSA; CAMPANHARO, SARAH CHAGAS; DA SILVA, AGNALDO FERNANDO BALDO; ASSANE, INACIO MATEUS; PILARSKI, FABIANA; SASANYA, JAMES JACOB; et al. Residue Depletion Profile and Estimation of Withdrawal Period for Sulfadimethoxine and Ormetoprim in Edible Tissues of Nile Tilapia (Oreochromis sp.) on Medicated Feed. ANIMALS, v. 13, n. 15, p. 13-pg., 2023-08-01. (21/03294-1, 19/06159-8, 16/16593-9, 18/05536-0, 21/08152-0, 19/22775-0, 12/18334-0)

NETO, PEDRO FRACCAROLLI; BALDO DA SILVA, AGNALDO FERNANDO; MORO, EVANDRO BILHA; PILARSKI, FABIANA; DE FREITAS, OSVALDO; MOONEY, MARK H.; RIZZATO PASCHOAL, JONAS AUGUSTO. Emamectin benzoate in tilapia: Alternative method for drug incorporation into feed and associated residual depletion study. Food Research International, v. 119, p. 524-529, MAY 2019. (12/18334-0, 14/50755-0)

CAMPANHARO, SARAH CHAGAS; DA SILVA, AGNALDO FERNANDO BALDO; BLEUZEN, ANAIS; DA SILVA, JONAS JOAQUIM MANGABEIRA; DE FREITAS, LUCAS VICTOR PEREIRA; ASSANE, INACIO MATEUS; PILARSKI, FABIANA; PASCHOAL, JONAS AUGUSTO RIZZATO. The association of modified QuEChERS and DLLME to offer high analytical detectability to assess residual depletion profile of erythromycin in fish. Food Chemistry, v. 405, p. 8-pg., 2023-03-30. (12/18334-0, 19/06159-8, 17/15052-7, 18/05536-0, 19/22775-0, 21/03294-1)