Low-Level Laser Therapy Reduces Lung Inflammation in an Experimental Model of Chronic Obstructive Pulmonary Disease Involving P2X7 Receptor

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Moraes, Gabriel da Cunha ; Vitoretti, Luana Beatriz ; de Brito, Aurileia Aparecida ; Alves, Cintia Estefano ; Rigonato de Oliveira, Nicole Cristine ; Dias, Alana dos Santos ; Teles Matos, Yves Silva ; Oliveira-Junior, Manoel Carneiro ; Franco Oliveira, Luis Vicente ; da Palma, Renata Kelly ; Candeo, Larissa Carbonera ; Lino-dos-Santos-Franco, Adriana ; Ratto Tempestine Horliana, Anna Carolina ; Gimenes, Joao Antonio Junior ; Aimbire, Flavio ; Vieira, Rodolfo Paula ; Ligeiro-de-Oliveira, Ana Paula

• Document type: Journal article
• Source: OXIDATIVE MEDICINE AND CELLULAR LONGEVITY; v. 2018, p. 8-pg., 2018-01-01.

Abstract

Chronic obstructive pulmonary disease (COPD) is a progressive disease characterized by irreversible airflow limitation, airway inflammation and remodeling, and enlargement of alveolar spaces. COPD is in the top five leading causes of deaths worldwide and presents a high economic cost. However, there are some preventive measures to lower the risk of developing COPD. Low-level laser therapy (LLLT) is a new effective therapy, with very low cost and no side effects. So, our objective was to investigate if LLLT reduces pulmonary alterations in an experimental model of COPD. C57BL/6 mice were submitted to cigarette smoke for 75 days (2x/day). After 60 days to smoke exposure, the treated group was submitted to LLLT (diode laser, 660 nm, 30 mW, and 3 J/cm(2)) for 15 days and euthanized for morphologic and functional analysis of the lungs. Our results showed that LLLT significantly reduced the number of inflammatory cells and the proinflammatory cytokine secretion such as IL-1 beta, IL-6, and TNF-alpha in bronchoalveolar lavage fluid (BALF). We also observed that LLLT decreased collagen deposition as well as the expression of purinergic P2X7 receptor. On the other hand, LLLT increased the IL-10 release. Thus, LLLT can be pointed as a promising therapeutic approach for lung inflammatory diseases as COPD. (AU)

• FAPESP's process: 12/16498-5 - Effect of low level laser therapy in experimental models of pulmonary chronic diseases
• Grantee: Ana Paula Ligeiro de Oliveira
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 14/14604-8 - Role of purinergic signaling in the antiinflammatory effects of aerobic training in a model of asthma induced by house dust mite
• Grantee: Manoel Carneiro de Oliveira Junior
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/23152-6 - Effect of low level laser therapy (LLLT) in an experimental model of pulmonary allergic inflammation induced house dust mite (HDM): study of dosimetry
• Grantee: Nicole Cristine Rigonato de Oliveira
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 15/13486-4 - Effect of low-level laser therapy on pulmonary inflammation in an experimental model of chronic obstructive pulmonary disease (COPD) induced by cigarette smoking: laser dosimetry studies
• Grantee: Cintia Estefano Alves
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 12/15165-2 - Role of purinergic signaling and of SOCS-JAK-STAT signaling in the antiinflammatory effects of aerobic training in experimental models of asma and in asthmatic individuals
• Grantee: Rodolfo de Paula Vieira
• Support Opportunities: Research Grants - Young Investigators Grants