| Full text | |
| Author(s): |
de Souza Mesquita, Leonardo Mendes
;
Bittencourt Rodrigues, Caroline Fabri
;
da Rocha, Claudia Quintino
;
Bianchim, Mayara Silveira
;
Rodrigues, Clenilson Martins
;
de Oliveira, Vanda Maria
;
Gaeta, Henrique Hessel
;
Belchor, Mariana Novo
;
Toyama, Marcos Hikari
;
Vilegas, Wagner
Total Authors: 10
|
| Document type: | Journal article |
| Source: | NATURAL PRODUCTS AND BIOPROSPECTING; v. 9, n. 1, p. 12-pg., 2019-02-01. |
| Abstract | |
We recognize the chemical composition of the acetonic extract of Rhizophora mangle barks (AERM) using mass spectrometry analysis [liquid chromatography (LC)-ESI-IT-MS/MS and matrix-assisted laser desorption/ionization-time of flight-MS (MALDI-TOF)]. Analgesic activity was evaluated by formalin and tail-flick experimental assays. Anti-inflammatory activity was performed by paw edema test induced by carrageenan and 48/80 compounds. The first series of experiments involved [LC]-FIA-IT-MS/MS with 11 separated catechins derivatives until degree of polymerization 3 (DP3). The spectra obtained by MALDI-TOF analysis of the AERM presented two homologous series: one based on polymers of m/z 288 Da increments (up to DP12) and another series based on polymers of m/z [288 + 162] Da increments (up to DP11). In addition to these series of flavan-3-ol, each DP had a subset of masses with a variation of - 16 Da (homologous series of afzelechins-m/z 873-3465 Da) and + 16 Da (homologous series of gallocatechins-m/z 905-3497 Da). A similar pattern with homologous series of gallocatechins and afzelechins could also be observed for a fifth and a sixth monohexoside series: glucogallocatechins (m/z 779-3371) and glucoafzelechins (m/z 747-3339). The intraperitoneal administration of different doses of AERM (50, 150 and 300 mu g mL(-1)) have a morphine-like effect and intense anti-inflammatory activity. (AU) | |
| FAPESP's process: | 09/52237-9 - Standardized herbal medicines for the treatment of chronic diseases |
| Grantee: | Wagner Vilegas |
| Support Opportunities: | BIOTA-FAPESP Program - Thematic Grants |
| FAPESP's process: | 14/23951-3 - Standardized phytotherapics for the treatment of chronicle diseases: Rhizophora mangle |
| Grantee: | Leonardo Mendes de Souza Mesquita |
| Support Opportunities: | Scholarships in Brazil - Master |