Nitric Oxide Releasing Polyamide Dendrimer with Anti-inflammatory Activity

Author(s)

Garzon-Porras, Ana M. ; Bertuzzi, Diego L. ; Lucas, Kurt ; da Silva, Laura C. E. ; de Oliveira, Marcelo G. ; Ornelas, Catia

• Document type: Journal article
• Source: ACS APPLIED POLYMER MATERIALS; v. 2, n. 5, p. 8-pg., 2020-05-01.

Abstract

Inflammatory processes are related to a wide variety of diseases with high global impact. Nitric oxide (NO) is an excellent candidate for the treatment of such diseases due to its important anti-inflammatory role in living systems, which is related to its concentration in the cellular medium that in turn depends directly on its release rate from NO releasing compounds. In this work, we synthesized a nitrate-terminated dendrimer and evaluated its anti-inflammatory properties. Remarkably, dendrimer bearing 18 NO-releasing groups was nontoxic, and exhibited anti-inflammatory activity (13.7-27.9% of IL-8 inhibition) throughout the tested concentrations (6.50 X 10(-2) to 4.17 X 10(3) nM of dendrimer; (1.17-7 SO x 10(4) nM of NO-moiety) On the other hand, the NO-releasing monomer was pro-inflammatory at concentrations higher than 62.5 nM. Investigation of the NO-releasing profiles from monomer and dendrimer using real-time NO analyzer NOA confirmed the slow release of NO from the dendrimer. Our results suggest that the NO release from the dendrimer occurs in a controlled fashion, keeping the anti-inflammatory effect of NO even at high concentrations. (AU)

• FAPESP's process: 18/02093-0 - Development of new nanomaterials for nanomedicine applications
• Grantee: Cátia Cristina Capêlo Ornelas Megiatto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/04929-0 - Development of biosensors for instantaneous detection of proteins and cancer cells, for application on on-site biopsy
• Grantee: Diego Luan Bertuzzi
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
• FAPESP's process: 18/14142-5 - Hybrid hydrogels for local and prolonged nitric oxide release
• Grantee: Laura Caetano Escobar da Silva
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 15/23761-2 - Development of new well-defined multifunctional dendrimers for applications in nanomedicine
• Grantee: Cátia Cristina Capêlo Ornelas Megiatto
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/02414-5 - Topical and absorbable biomaterials for the local nitric oxide release
• Grantee: Marcelo Ganzarolli de Oliveira
• Support Opportunities: Research Projects - Thematic Grants