Validation of a method for simultaneous quantification of total carotenes and tocols in vegetable oils by HPLC

Author(s)

Silva, Simone M. ^[1] ; Rocco, Silvana Aparecida ^[2] ; Sampaio, Klicia Araujo ^[1] ; Taham, Thiago ^[1] ; Meller da Silva, Luiza Helena ^[3] ; Ceriani, Roberta ^[4] ; Meirelles, Antonio J. A. ^[1]

Affiliation

^[1] Univ Estadual Campinas, Sch Food Engn, Dept Food Engn, EXTRAE, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Sch Med, Dept Internal Med, Campinas, SP - Brazil

^[3] Fed Univ Para, UFPA, Fac Food Engn, Belem, PA - Brazil

^[4] Univ Estadual Campinas, Sch Chem Engn, Dept Chem Proc, Campinas, SP - Brazil

• Document type: Journal article
• Source: Food Chemistry; v. 129, n. 4, p. 1874-1881, DEC 15 2011.
• Web of Science Citations: 18

Abstract

A normal-phase HPLC method for analysis of carotenes, tocopherols and tocotrienols has been developed and validated. In this work we presented a modification to the official AOCS method for analysis of tocols which allowed simultaneous quantification of the three groups of compounds, including carotenes. Analytes were separated using a gradient mobile phase (hexane and isopropanol) and with a gradient flow rate (1-2 mL min(-1)). The column effluent was monitored by Photo Diode Array detector (PDA) set at 292 nm (tocols) and 455 nm (beta-carotene) and by fluorescence detector set at an excitation wavelength of 290 and 330 nm emission. Inter- and intra-run accuracies and precision of the analytical method were better than +/- 15%. The lower limit of quantification was 5.0 mg L(-1) for the tocols and 0.1 mg L(-1) for carotenes. The method has been applied for the quantification of these compounds in Amazon oils. (C) 2011 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 10/16634-0 - Separation processes by stripping in the fat and oil industry
• Grantee: Roberta Ceriani
• Support type: Regular Research Grants