Cytotoxic Effect of Niobium Phosphate Glass-based Gutta-Percha Points on Periodontal Ligament Fibroblasts In Vitro

Author(s)

Bosio Meneses, Claudia Caroline ; Olivi, Lucas Tofanello ; Carvalho, Ceci Nunes ; Gavini, Giulio ; Sipert, Carla Renata

• Document type: Journal article
• Source: JOURNAL OF ENDODONTICS; v. 46, n. 9, p. 5-pg., 2020-09-01.

Abstract

Introduction: The outcome of root canal obturation might be affected by the chemical components of the chosen filling materials. Niobium phosphate glass-based gutta-percha (GNB) was proposed as a biomaterial-based obturation point. This study aimed to investigate the cytotoxic and cell modulation effects of GNB points on human periodontal ligament fibroblasts (PDLFs) in vitro. Methods: Human PDLFs were cultured for the assays. Extracts of regular gutta-percha (GP) points and GNB were obtained, serially diluted (1:5, 1:10, and 1:25), and used to stimulate PDLFs. A cell viability assay was performed using alamarBlue reagent (Molecular Probes, Waltham, MA), and reverse transcription quantitative polymerase chain reaction was used to assess the gene expression for collagen type I and cementum protein 1. One-way analysis of variance followed by the Tukey post hoc test was performed (P < .05). Results: Regular GP reduced cell viability only in pure extracts, whereas GNB exhibited cytotoxicity to PDLFs in pure extracts as well as 1/5 and 1/10 dilutions. The gene expression of collagen type I was down-regulated only in the GNB group (P < .05). The expression of cementum protein 1 remained unaltered by both tested materials. Conclusions: The addition of niobium phosphate glass to GP points increased cytotoxicity, affecting PDLF viability and partially disturbing physiological cell function. (AU)

• FAPESP's process: 12/18220-4 - Effect of composite and bioactive niobophosphate glass on biofilm formation
• Grantee: Ceci Nunes Carvalho
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 17/00643-0 - Effect of gutta-percha on in vitro biomodulation of periodontal ligament fibroblasts
• Grantee: Lucas Tofanello Olivi
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 17/01737-8 - Role of endocanabinoids on the modulation of human apical papilla cells in vitro
• Grantee: Claudia Caroline Bosio Meneses
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 16/13944-5 - Role of inflammatory mediators on the modulation of Cells from Apical Papilla: in vitro study
• Grantee: Carla Renata Sipert
• Support Opportunities: Regular Research Grants