Analysis of methodologies for evaluating the solubility of calcium silicate-based sealer Bio-C Sealer

Author(s)

Mário TANOMARU-FILHO ^[1] ; Alessandra Alves GONÇALVES ^[2] ; Airton Oliveira SANTOS-JUNIOR ^[3] ; Karina Ines Medina Carita TAVARES ^[4] ; Jáder Camilo PINTO ; Juliane Maria GUERREIRO-TANOMARU ^[6]

Affiliation

^[1] Universidade Estadual Paulista Júlio de Mesquita Filho. Faculdade de Odontologia de Araraquara. Departamento de Odontologia Restauradora - Brasil

^[2] Universidade Estadual Paulista Júlio de Mesquita Filho. Faculdade de Odontologia de Araraquara. Departamento de Odontologia Restauradora - Brasil

^[3] Universidade Estadual Paulista Júlio de Mesquita Filho. Faculdade de Odontologia de Araraquara. Departamento de Odontologia Restauradora - Brasil

^[4] Universidade Estadual Paulista Júlio de Mesquita Filho. Faculdade de Odontologia de Araraquara. Departamento de Odontologia Restauradora - Brasil

^[6] Universidade Estadual Paulista Júlio de Mesquita Filho. Faculdade de Odontologia de Araraquara. Departamento de Odontologia Restauradora - Brasil

• Document type: Journal article
• Source: Rev. odontol. UNESP; v. 52, 2023-12-04.

Abstract

Abstract Introduction Calcium silicate-based sealers demonstrate greater solubility in distilled water. The use of alternative methodologies can help at better understanding the solubility of these materials. Objective To evaluate the effect of the immersion solution and the type of experimental model on the solubility of ready-to-use Bio-C Sealer. Material and method Circular models of polyethylene or bovine dentin (n = 16) were made. After insertion of the sealer, the specimens were kept in an oven at 37°C and 95% humidity for 48h. Subsequently, the samples were weighed on a precision balance to determine the initial mass. Next, the specimens were immersed in 7.5 mL of distilled water (pH 6.5) or PBS (pH 7.0) (n = 8) for 28 days. After that, the samples were removed from the solutions and weighed every 24 hours until the final mass stabilized (0.001g). Test specimens made with Bio-C Sealer were used as a control. Solubility was evaluated according to the difference between the initial and final mass in percentage. Two-Way ANOVA test and Tukey post-hoc tests were performed (α=0.05). Result Immersion in distilled water provided greater solubility compared to PBS regardless of the experimental model (p<0.05). The test specimens showed greater solubility, followed by polyethylene and dentin models immersed in distilled water (p<0.05). There was no difference between the experimental models immersed in PBS (p>0.05). Conclusion Bio-C Sealer presents significantly greater solubility in distilled water than in PBS under all conditions. Experimental model using bovine dentin and PBS as an immersion solution demonstrates reduction in the mass loss of Bio-C Sealer and can be a valuable alternative for evaluating the solubility of bioceramic sealers. (AU)

• FAPESP's process: 21/11496-3 - Synthesis of new reparative biomaterial, evaluation of calcium silicate materials and analysis of the effect of the application of bioglass in the treatment of dentin and in the coating of Gutta percha in the interaction with bioceramic root canal sealers
• Grantee: Mario Tanomaru Filho
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/11011-7 - Physicochemical and biological characteristics of clinker with different particle sizes besides root repair cements and root canal sealers based on calcium silicate
• Grantee: Karina Ines Medina Carita Tavares
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 20/11012-3 - Comparative evaluation between micro-CT and CBCT using the e-Vol DX software in the analysis of obturation in oval canals and dentinal thickness in MB1 and MB2 canals of maxillary molars after preparation and retreatment
• Grantee: Airton Oliveira Santos Junior
• Support Opportunities: Scholarships in Brazil - Doctorate