A new proposal for evaluating of the solubility of bioceramic materials in dentin tubes after immersion in PBS: a laboratory investigation

Author(s)

Giovanna da Cunha MENDONÇA ^[1] ; Karina Ines Medina Carita TAVARES ^[2] ; Airton Oliveira SANTOS-JUNIOR ^[3] ; Jáder Camilo PINTO ; Juliane Maria GUERREIRO-TANOMARU ^[5] ; Mário TANOMARU-FILHO ^[6]

Affiliation

^[1] Universidade Estadual Paulista Júlio de Mesquita Filho. Faculdade de Odontologia de Araraquara. Departamento de Odontologia Restauradora - Brasil

^[2] Universidade Estadual Paulista Júlio de Mesquita Filho. Faculdade de Odontologia de Araraquara. Departamento de Odontologia Restauradora - Brasil

^[3] Universidade Estadual Paulista Júlio de Mesquita Filho. Faculdade de Odontologia de Araraquara. Departamento de Odontologia Restauradora - Brasil

^[5] Universidade Estadual Paulista Júlio de Mesquita Filho. Faculdade de Odontologia de Araraquara. Departamento de Odontologia Restauradora - Brasil

^[6] Universidade Estadual Paulista Júlio de Mesquita Filho. Faculdade de Odontologia de Araraquara. Departamento de Odontologia Restauradora - Brasil

• Document type: Journal article
• Source: Rev. odontol. UNESP; v. 52, 2023-12-04.

Abstract

Abstract Introduction Repair materials must have low solubility. Phosphate buffered saline (PBS) allows simulating clinical condition and interaction with dentin may be important for the correct evaluate of mass loss of bioceramic cements. Objective To evaluate the effect of distilled water (DW), or PBS immersion on the solubility of Bio-C Repair (BCR, Angelus) or MTA Repair HP (MTAHP, Angelus) using a dentin tube model. Material and method Bovine dentin tubes with a length of 4 mm, an internal diameter of 1.5 mm and walls thickness of approximately 1 mm were made. The specimens were immersed in DW for 24h, then filled with BCR or MTAHP (n = 14) and stored in an oven at 37°C and 95% humidity for 24h. After being weighed on a precision balance to determine the initial mass, the specimens were immersed in DW (pH 6.5) or PBS (pH 7.0) (n = 7) for 28 days. Empty tubes also were used for calculating the mass loss of the dentin (n=4). After this period, the specimens were weighed until stabilization of the final mass occurred (0.001g). The solubility of each material was evaluated. ANOVA and Tukey statistical tests were performed (α=0.05). Result BCR and MTAHP showing gain of mass in DW and mass loss in PBS (p<0.05). Conclusion The immersion solution influenced the solubility of BCR and MTAHP using dentin tube model. The new methodological proposal could be an alternative to ISO standards for testing the solubility of bioceramic cements. (AU)

• FAPESP's process: 21/11496-3 - Synthesis of new reparative biomaterial, evaluation of calcium silicate materials and analysis of the effect of the application of bioglass in the treatment of dentin and in the coating of Gutta percha in the interaction with bioceramic root canal sealers
• Grantee: Mario Tanomaru Filho
• Support Opportunities: Regular Research Grants
• FAPESP's process: 20/11011-7 - Physicochemical and biological characteristics of clinker with different particle sizes besides root repair cements and root canal sealers based on calcium silicate
• Grantee: Karina Ines Medina Carita Tavares
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 20/11012-3 - Comparative evaluation between micro-CT and CBCT using the e-Vol DX software in the analysis of obturation in oval canals and dentinal thickness in MB1 and MB2 canals of maxillary molars after preparation and retreatment
• Grantee: Airton Oliveira Santos Junior
• Support Opportunities: Scholarships in Brazil - Doctorate