A novel A2 allele found in Leishmania (Leishmania) infantum chagasi

Author(s)

Trícia Maria Ferreira de Sousa Oliveira ^[1] ; Elton José Rosas de Vasconcelos ^[2] ; Andréa Cristina Higa Nakaghi ^[3] ; Tânia Paula Aquino Defina ^[4] ; Márcia Mariza Gomes Jusi ^[5] ; Cristiane Divan Baldani ^[6] ; Ângela Kaysel Cruz ^[7] ; Rosangela Zacarias Machado ^[8]

Affiliation

^[1] Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos. Departamento de Zootecnia

^[2] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos

^[3] Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinária. Departamento de Patologia Veterinária

^[4] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos

^[5] Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinária. Departamento de Patologia Veterinária

^[6] Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária

^[7] Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Biologia Celular e Molecular e Bioagentes Patogênicos

^[8] Universidade Estadual Paulista. Faculdade de Ciências Agrárias e Veterinária. Departamento de Patologia Veterinária

• Document type: Journal article
• Source: Revista Brasileira de Parasitologia Veterinária; v. 20, n. 1, p. 42-48, 2011-03-00.

Abstract

Visceral leishmaniasis (VL) is a widely spread zoonotic disease. In Brazil the disease is caused by Leishmania (Leishmania) infantum chagasi. Peridomestic sandflies acquire the etiological agent by feeding on blood of infected reservoir animals, such as dogs or wildlife. The disease is endemic in Brazil and epidemic foci have been reported in densely populated cities all over the country. Many clinical features of Leishmania infection are related to the host-parasite relationship, and many candidate virulence factors in parasites that cause VL have been studied such as A2 genes. The A2 gene was first isolated in 1994 and then in 2005 three new alleles were described in Leishmania (Leishmania) infantum. In the present study we amplified by polymerase chain reaction (PCR) and sequenced the A2 gene from the genome of a clonal population of L. (L.) infantum chagasi VL parasites. The L. (L.) infantum chagasi A2 gene was amplified, cloned, and sequenced in. The amplified fragment showed approximately 90% similarity with another A2 allele amplified in Leishmania (Leishmania) donovani and in L.(L.) infantum described in literature. However, nucleotide translation shows differences in protein amino acid sequence, which may be essential to determine the variability of A2 genes in the species of the L. (L.) donovani complex and represents an additional tool to help understanding the role this gene family may have in establishing virulence and immunity in visceral leishmaniasis. This knowledge is important for the development of more accurate diagnostic tests and effective tools for disease control. (AU)