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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

3D culture applied to reproduction in females: possibilities and perspectives

Full text
Author(s):
Giuliana de Avila Ferronato [1] ; Franciele Flores Vit [2] ; Juliano Coelho da Silveira [3]
Total Authors: 3
Affiliation:
[1] Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos - Brasil
[2] Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos - Brasil
[3] Universidade de São Paulo. Faculdade de Zootecnia e Engenharia de Alimentos - Brasil
Total Affiliations: 3
Document type: Journal article
Source: Animal Reproduction; v. 21, n. 1 2024-03-08.
Abstract

Abstract In vitro cell culture is a well-established technique present in numerous laboratories in diverse areas. In reproduction, gametes, embryos, and reproductive tissues, such as the ovary and endometrium, can be cultured. These cultures are essential for embryo development studies, understanding signaling pathways, developing drugs for reproductive diseases, and in vitro embryo production (IVP). Although many culture systems are successful, they still have limitations to overcome. Three-dimensional (3D) culture systems can be close to physiological conditions, allowing greater interaction between cells and cells with the surrounding environment, maintenance of the cells' natural morphology, and expression of genes and proteins such as in vivo. Additionally, three-dimensional culture systems can stimulated extracellular matrix generating responses due to the mechanical force produced. Different techniques can be used to perform 3D culture systems, such as hydrogel matrix, hanging drop, low attachment surface, scaffold, levitation, liquid marble, and 3D printing. These systems demonstrate satisfactory results in follicle culture, allowing the culture from the pre-antral to antral phase, maintaining the follicular morphology, and increasing the development rates of embryos. Here, we review some of the different techniques of 3D culture systems and their applications to the culture of follicles and embryos, bringing new possibilities to the future of assisted reproduction. (AU)

FAPESP's process: 22/02701-5 - Generation of microfluidic devices with different geometries for in vitro fertilization
Grantee:Franciele Flores Vit
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 21/06645-0 - Extracellular vesicles as a platform for diagnostic and manipulation of the in vitro embryo production system: the next generation in animal reproduction
Grantee:Juliano Coelho da Silveira
Support Opportunities: Research Grants - Young Investigators Grants - Phase 2
FAPESP's process: 19/25675-7 - In vitro production of bovine embryos in different 3D culture systems: effects on nuclear morphology, methylation pattern, gene transcription and extracellular vesicles
Grantee:Giuliana de Avila Ferronato
Support Opportunities: Scholarships in Brazil - Master