| Full text | |
| Author(s): |
Okuro, Paula K.
;
Viau, Michele
;
Marze, Sebastien
;
Laurent, Sophie
;
Cunha, Rosiane L.
;
Berton-Carabin, Claire
;
Meynier, Anne
Total Authors: 7
|
| Document type: | Journal article |
| Source: | FOOD & FUNCTION; v. 14, n. 24, p. 14-pg., 2023-11-21. |
| Abstract | |
Investigating the gastrointestinal fate of food emulsions is critical to unveil their nutritional relevance. To this end, the protocol standardized by COST INFOGEST 2.0 is meaningful for guiding in vitro digestion experiments. In contrast with studies addressing emulsions with low dispersed phase volume fraction (phi 0.05-0.1), we presently raise some points for a proper interpretation of the digestibility of emulsions with high lipid content using the pH-stat method. Oil-in-water high internal phase emulsions (HIPEs) were submitted to gastric pre-lipolysis with the addition of rabbit gastric lipase (RGE). Commercial mayonnaise (phi 0.76) was systematically diluted (phi 0.025, 0.05, 0.1, 0.15, 0.25, 0.4, and 0.76) to cover a wide range of enzyme-to-lipid ratios (8.5-0.3 U per mu mol for RGE and 565.1-18.6 U per mu mol for pancreatin, in the gastric and intestinal phases, respectively). Lipolysis was tracked either by fatty acid titration (NaOH titration) or completed by analysis of lipid classes and fatty acid composition. Gastric lipase resulted in substantial lipid hydrolysis, reaching 20 wt% at low lipid fractions (phi 0.025 and 0.05). Likewise, the kinetics and extent of lipolysis during intestinal digestion were modulated by the enzyme-to-substrate ratio. A logarithmic relationship between lipid hydrolysis and lipid concentration was observed, with a very limited extent at the highest lipid content (phi 0.76). A holistic interpretation relying on FFA titration and further evaluation of all lipolytic products appears of great relevance to capture the complexity of the effects involved. Overall, this work contributes to rationally and critically evaluating the outcomes of static in vitro experiments of lipid digestion. (AU) | |
| FAPESP's process: | 19/27354-3 - Architecture of colloidal delivery systems: what is the role of structure on the digestibility? |
| Grantee: | Rosiane Lopes da Cunha |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 19/26046-3 - Investigation of high internal phase emulsions-HIPEs as strategy design to tailor in vitro lipid digestion and bioaccessibility of bioactive compound |
| Grantee: | Paula Kiyomi Okuro |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |
| FAPESP's process: | 18/20308-3 - Designing oil structured colloidal systems towards enhanced bioavailability of bioactive compounds on in vitro digestibility behavior |
| Grantee: | Paula Kiyomi Okuro |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |