PAPP-A enhances the antioxidative effects of IGF-1 during bovine in vitro embryo production

Author(s)

Franchi, Fernanda Fagali ; Santos, Priscila Helena dos ; Fontes, Patricia Kubo ; Quaglio, Ana Elisa Valencise ; Nunes, Sarah Gomes ; Mingoti, Gisele Zoccal ; Castilho, Anthony Cesar de Souza

• Document type: Journal article
• Source: Theriogenology; v. 229, p. 11-pg., 2024-08-27.

Abstract

We investigated whether exogenous pregnancy-associated plasma protein-A (PAPP-A) enhances the antioxidant role of insulin-like growth factor-1 (IGF-1) in bovine in vitro embryo production (IVP). We performed standard in vitro maturation (IVM) and in vitro culture (IVC) or added menadione to promote an oxidative stressed microenvironment and evaluated the antioxidant effect of IGF-1 alone or in combination with PAPP-A (IGF-1/PAPPA). In IVM, the treatments did not affect oocyte nuclear development, total GSH content, cumulus cell gene expression, and blastocyst yield. Nevertheless, IGF-1/PAPP-A treatment prevented an increase in reactive oxygen species (ROS) and mitochondrial membrane potential (MMP) levels. In IVC, the treatments did not affect the total GSH content on blastocysts and IVC media, but IGF-1 and IGF-1/PAPP-A treatments increased blastocyst yield compared to the menadione group. In addition, IGF-1/PAPP-A treatment had lower ROS levels and regulated genes related to embryonic quality compared to the control and menadione groups. Overall, we showed that PAPP-A could enhance the antioxidant role of IGF-1 during IVP in cattle by avoiding higher ROS levels in oocytes and blastocysts and modulating the transcriptional abundance of genes involved in oxidative protection and embryonic quality. (AU)

• FAPESP's process: 18/06674-7 - Impact of estradiol on 3D culture of bovine oviduct epithelial cells and its effects on in vitro oviduct-embryo communication
• Grantee: Anthony César de Souza Castilho
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/04505-5 - Characterization of microRNAs (miRNAs) in bovine antral follicles from Nelore cows: involvement during ovarian follicle cells differentiation and regulation of LH receptor (LHR) expression
• Grantee: Anthony César de Souza Castilho
• Support Opportunities: Multi-user Equipment Program
• FAPESP's process: 13/11480-3 - Characterization of microRNAs (miRNAs) in bovine antral follicles from Nelore cattle: involvement during ovarian follicle cells differentiation and regulation of LH receptor (LHr) expression
• Grantee: Anthony César de Souza Castilho
• Support Opportunities: Research Grants - Young Investigators Grants