In Situ Biofabrication of Microbial Cellulose Capsules Carrying Cubosomes: Toward Colon Targeted Multidrug Delivery

Author(s):Show less -

Ferreira, Filipe V. ; Ezazi, Nazanin Z. ; Otoni, Caio G. ; Aguiar, Ana Carolina ; Bianchi, Jhonatan R. O. ; Lopes, Joao H. ; dos Santos, Danilo M. ; Greca, Luiz G. ; Barud, Hernane S. ; Santos, Helder A. ; Rojas, Orlando J. ; Mattoso, Luiz Henrique Capparelli

• Document type: Journal article
• Source: ACS APPLIED POLYMER MATERIALS; v. 6, n. 7, p. 13-pg., 2024-04-01.

Abstract

The colon is a main absorption site (nutrients and drugs) and a target for oral therapeutic delivery. However, the latter is challenged by the fact that most drugs degrade during transit in the gastrointestinal tract (GIT). Herein, we rationally designed a universal controlled-release system based on cubosomes contained in microbial nanocellulose capsules that enabled oral administration and pH-triggered delivery of bioactives. We show that the bicontinuous cubosome structure allows the simultaneous incorporation of drugs with differing polarity or surface energy. Furthermore, the multidrug cubosomes combined with the cellulose carrier by in situ biofabrication was demonstrated as a route toward multicomponent 3D capsules with added protection in the GIT. The obtained capsules were subsequently coated with sodium alginate to enable responsiveness, achieving dual cargo-controlled release and site-specific administration. In sum, we successfully engineered pH-responsive, nontoxic microcapsules as a versatile platform for colon-targeted multidrug delivery. (AU)

• FAPESP's process: 22/03247-6 - Investigation of mesoporous nanoparticles from bioactive glasses containing niobium in bone tissue engineering
• Grantee: João Henrique Lopes
• Support Opportunities: Research Grants - Initial Project
• FAPESP's process: 20/07956-6 - Engineered biofabrication of biocellulose microcapsules containing arabinogalactan from biorefinery and cubosomes for colon-targeted delivery of drugs
• Grantee: Filipe Vargas Ferreira
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 17/20973-4 - Coaxial electrospun nanofibers based on chitosan for controlled release of antibiotics and periodontic lesion treatment
• Grantee: Danilo Martins dos Santos
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 21/12071-6 - Tailoring colloids through supramolecular interactions: from fundamentals to applications
• Grantee: Watson Loh
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
• Grantee: Roberto de Campos Giordano
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants
• FAPESP's process: 21/00980-1 - Synthesis of nanocellulose capsules for oral insulin administration
• Grantee: Ana Carolina de Aguiar
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 18/22214-6 - Towards a convergence of technologies: from sensing and biosensing to information visualization and machine learning for data analysis in clinical diagnosis
• Grantee: Osvaldo Novais de Oliveira Junior
• Support Opportunities: Research Projects - Thematic Grants