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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biotransformation using Mucor rouxii for the production of oleanolic acid derivatives and their antimicrobial activity against oral pathogens

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Author(s):
Capel, Clarissa S. [1] ; de Souza, Ana C. D. [1] ; de Carvalho, Tatiane C. [1] ; de Sousa, Joao P. B. [1] ; Ambrosio, Sergio R. [2] ; Martins, Carlos H. G. [2] ; Cunha, Wilson R. [2] ; Galan, Rosario H. [3] ; Furtado, Niege A. J. C. [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Ribeirao Preto, SP - Brazil
[2] Univ Franca, Nucleo Pesquisas Ciencias Exatas & Tecnol, BR-14404600 Franca, SP - Brazil
[3] Univ Cadiz, Fac Ciencias, Puerto Real 11510 - Spain
Total Affiliations: 3
Document type: Journal article
Source: Journal of Industrial Microbiology & Biotechnology; v. 38, n. 9, p. 1493-1498, SEP 2011.
Web of Science Citations: 15
Abstract

The goal of this study is to produce oleanolic acid derivatives by biotransformation process using Mucor rouxii and evaluate their antimicrobial activity against oral pathogens. The microbial transformation was carried out in shake flasks at 30A degrees C for 216 h with shaking at 120 rpm. Three new derivatives, 7 beta-hydroxy-3-oxo-olean-12-en-28-oic acid, 7 beta,21 beta-dihydroxy-3-oxo-olean-12-en-28-oic acid, and 3 beta,7 beta,21 beta-trihydroxyolean-12-en-28-oic acid, and one know compound, 21 beta-hydroxy-3-oxo-olean-12-en-28-oic acid, were isolated, and the structures were elucidated on the basis of spectroscopic analyses. The antimicrobial activity of the substrate and its transformed products was evaluated against five oral pathogens. Among these compounds, the derivative 21 beta-hydroxy-3-oxo-olean-12-en-28-oic acid displayed the strongest activity against Porphyromonas gingivalis, which is a primary etiological agent of periodontal disease. In an attempt to improve the antimicrobial activity of the derivative 21 beta-hydroxy-3-oxo-olean-12-en-28-oic acid, its sodium salt was prepared, and the minimum inhibitory concentration against P. gingivalis was reduced by one-half. The biotransformation process using M. rouxii has potential to be applied to the production of oleanolic acid derivatives. Research and antimicrobial activity evaluation of new oleanolic acid derivatives may provide an important contribution to the discovery of new adjunct agents for treatment of dental diseases such as dental caries, gingivitis, and periodontitis. (AU)

FAPESP's process: 05/59329-5 - Studies on the biotransformation of pentacyclic triterpenes by filamentous fungi and evaluation of the antitumoral and trypanocide activities of the derivatives
Grantee:Niege Araçari Jacometti Cardoso Furtado
Support Opportunities: BIOTA-FAPESP Program - Young Investigators Grants