Lipidomics by Nuclear Magnetic Resonance Spectroscopy and Liquid Chromatography-High-Resolution Mass Spectrometry in Osteosarcoma: A Pilot Study

Author(s):Show less -

Pontes, Joao Guilherme de Moraes ; Jadranin, Milka ; Assalin, Marcia Regina ; Escobar, Melissa Quintero ; Stanisic, Danijela ; Costa, Tassia Brena Barroso Carneiro ; Lengert, Andre van Helvoort ; Boldrini, Erica ; da Silva, Sandra Regina Morini ; Vidal, Daniel Onofre ; Liu, Leticia Huan Bacellar ; Maschietto, Mariana ; Tasic, Ljubica

• Document type: Journal article
• Source: METABOLITES; v. 14, n. 8, p. 17-pg., 2024-08-01.

Abstract

Cancer is a complex disease that can also affect the younger population; however, it is responsible for a relatively high mortality rate of children and youth, especially in low- and middle-income countries (LMICs). Besides that, lipidomic studies in this age range are scarce. Therefore, we analyzed blood serum samples from young patients (12 to 35 years) with bone sarcoma (osteosarcoma) and compared their lipidomics to the ones from the control group of samples, named healthy control (HC group), using NMR and LC-MS techniques. Furthermore, differences in the lipidomic profiles between OS patients with and without metastasis indicate higher glycerophosphocholine (GPC) and glycerophospholipid (GPL) levels in osteosarcoma and increased cholesterol, choline, polyunsaturated fatty acids (PUFAs), and glycerols during the metastasis. These differences, detected in the peripheral blood, could be used as biomarkers for liquid biopsy. (AU)

• FAPESP's process: 18/21047-9 - Childhood cancer: genetic predisposition and mechanisms of origin
• Grantee: Ana Cristina Victorino Krepischi
• Support Opportunities: Regular Research Grants
• FAPESP's process: 23/03780-9 - Metabonomics and Metabolomics of the exposome by NMR
• Grantee: João Guilherme de Moraes Pontes
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 18/24069-3 - ReSEARCH: Recognizing Signatures of the Exposome to Anticipate the Risks for a Continuous Health
• Grantee: Fernando Barbosa Júnior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/06510-4 - Application of Nuclear Magnetic Resonance (NMR) in metabolomics and metabonomics of human diseases
• Grantee: Ljubica Tasic
• Support Opportunities: Regular Research Grants