Mucoadhesive liquid crystal precursor system for photodynamic therapy of oral cancer mediated by methylene blue

Author(s)

Balian, Giovana Maria Fioramonti Calixto ; Luiz, Marcela Tavares ; Di Filippo, Leonardo Delello ; Chorilli, Marlus

• Document type: Journal article
• Source: Photodiagnosis and Photodynamic Therapy; v. 44, p. 8-pg., 2023-10-03.

Abstract

Oral cancer is one of the most prevalent types of cancer head and neck cancers worldwide. Photodynamic therapy (PDT) has demonstrated great potential against cancers, reducing long-term morbidity. In this study, we investigated the incorporation of methylene blue (MB) in a mucoadhesive liquid crystal precursor system (LCPS) for oral cancer treatment. The photostability and the in vitro release, permeation, and retention profile of MB loaded LCPS (MB-LCPS) were investigated, as well as its in vitro PDT activity against normal (HaCaT) and tumoral (HSC-3) cell lines. LCPS increased the photostability of MB and exhibited a prolonged release profile of MB. In addition, LCPS increased the retention of MB in the porcine esophageal mucosa by around 3 times higher than the MB solution. The retention of MB in LCPS was around 2 times greater than its permeability, which is suitable for guaranteeing the maintenance of the therapy in the oral cavity. In vitro cytotoxicity assay indicated that MB-LCPS increased the antitumoral activity of MB after 20 min of irradiation at 660 nm and 12.5 J/cm2. The results obtained suggest that the developed formulation is an interesting strategy for the potential application in the treatment of oral cancer by PDT. (AU)

• FAPESP's process: 13/01565-1 - Mucoadhesive precursor systems of liquids crystals for buccal admnistration of antigelatinolytic peptide associated with photodynamic therapy in the treatment of oral cancer
• Grantee: Giovana Maria Fioramonti Calixto Balian
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/50928-2 - INCT 2014: Pharmaceutical Nanotechnology: a transdisciplinary approach
• Grantee: Maria Vitória Lopes Badra Bentley
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 16/06337-5 - Evaluation of the effects of CTT1-loaded liquid crystalline system associated to photodynamic therapy in tumor growth using chorioallantoic membrane
• Grantee: Giovana Maria Fioramonti Calixto Balian
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate
• FAPESP's process: 21/04007-6 - Evaluation of epirubicin-loaded trastuzumabe-modified immunoliposomes and imunomagnetoliposomes in the breast cancer therapy
• Grantee: Marcela Tavares Luiz
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 19/07245-5 - Lidocaine and epinephrine co-loaded mucoadhesive liquid crystal precursor systems as a strategy for topical anesthesia in the buccal cavity
• Grantee: Giovana Maria Fioramonti Calixto Balian
• Support Opportunities: Scholarships in Brazil - Post-Doctoral