A multi-technique approach for nanoherbicide tracking: uptake and translocation pathways of the metribuzin nanocarrier in weed plants

Author(s):Show less -

Takeshita, Vanessa ; Preisler, Ana Cristina ; Munhoz-Garcia, Gustavo Vinicios ; Carvalho, Lucas Braganca ; Pinacio, Camila de Werk ; Oliveira, Halley Caixeta ; Tornisielo, Valdemar Luiz ; Cardoso, Brian Cintra ; Ramalho, Eduardo Fonte Boa ; Pimpinato, Rodrigo Floriano ; Dionisio, Amanda Martins ; Verri Jr, Waldiceu Aparecido ; Fraceto, Leonardo Fernandes

• Document type: Journal article
• Source: ENVIRONMENTAL SCIENCE-NANO; v. 11, n. 11, p. 15-pg., 2024-09-03.

Abstract

Understanding the strategies of delivering active ingredients with nanoparticles to plant species is crucial to implement a safe-by-design approach for pesticides. Here, we used metribuzin (MTZ) as a study model to understand the plant uptake and distribution pathways of polymeric nanocarriers. We investigated the weed-control efficacy, uptake, internalization, and distribution of the (nano) herbicide MTZ in Amaranthus viridis (C4 species) and Bidens pilosa (C3 species) after soil and foliar application. A radiolabeled herbicide and a fluorescent probe were used as complementary tools to track both MTZ and nanoparticles in plants. The weed-control results indicated significant dose reductions with MTZ nanoencapsulation (from 1/2 to 10-fold doses). Root uptake was an efficient pathway for A. viridis and the B. pilosa entry of nanoMTZ occurred preferentially in the stomata and was internalized in leaf mesophyll cells. No differences in herbicide uptake were observed in the soil, and the nanoMTZ distribution was 1.3-1.5 lower than MTZ. After foliar application, nanoMTZ was absorbed 2.5 times more than MTZ in A. viridis and was similar in B. pilosa. NPs were concentrated in the vascular cells after soil application and in the stomata entrance after foliar application. For B. pilosa, the internalization in the leaf mesophyll was more evident than that in A. viridis. Our findings indicated that consideration of the target weed species and application mode will increase the efficacy of control using nanoformulations. A multi-technique approach indicated that the target weed species and application mode can determine the control efficacy and uptake of PCL nanoparticles, and the distribution of the metribuzin herbicide in plants, depending on NP-plant interactions. (AU)

• FAPESP's process: 22/12221-0 - Metribuzin nanoformulations uptake and distribution in target plants in different soil system
• Grantee: Camila de Werk Pinácio
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• FAPESP's process: 17/21004-5 - Agriculture, micro/nanotechnology and environment: from evaluation of the mechanisms of action to studies of transport and toxicity
• Grantee: Leonardo Fernandes Fraceto
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 21/10639-5 - Center for Research on Biodiversity Dynamics and Climate Change
• Grantee: Leonor Patricia Cerdeira Morellato
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 19/04758-1 - Nanoparticles as a carrier of herbicides applied in pre-emergency system in soils derived from no-tillage and conventional
• Grantee: Vanessa Takeshita
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 21/03455-5 - Straw-herbicide interaction in no-tillage system: effects of pre-emergent herbicides on the control of slender amaranth
• Grantee: Camila de Werk Pinácio
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 22/01554-9 - Impacts of metribuzin nanoformulation application on soil non-target organisms
• Grantee: Brian Cintra Cardoso
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 22/16554-4 - Application of radiometric techniques to characterize the retention, mobility, toxicity, and degradation of herbicide nanocarrier systems in the environment
• Grantee: Valdemar Luiz Tornisielo
• Support Opportunities: Regular Research Grants
• FAPESP's process: 22/12200-3 - Tracking delivery of metribuzin nanoformulations using fluorescent markers
• Grantee: Brian Cintra Cardoso
• Support Opportunities: Scholarships abroad - Research Internship - Scientific Initiation
• FAPESP's process: 22/00718-8 - Glyphosate carrier nanosystems: from development to application in weed management
• Grantee: Gustavo Vinícios Munhoz Garcia
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 22/00509-0 - Distribution and effects of metribuzin nanoparticles on soil, soil communities and plants in a soil mesocosm experiment: a comparison of two polymeric nanocarrier systems
• Grantee: Vanessa Takeshita
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate