A study of the oral bioavailability and biodistribution increase of Nanoencapsulation-driven Delivering radiolabeled anthocyanins

Author(s)

Rosales, Thiecla Katiane Osvaldt ; da Silva, Fabio Fernando Alves ; Rivera, Andy Gonzalez ; dos Santos, Sofia Nascimento ; Bustos, Daniel ; Morales-Quintana, Luis Alberto ; Santos, Helder A. ; Bernardes, Emerson Soares ; Fabi, Joao Paulo

• Document type: Journal article
• Source: Food Research International; v. 197, p. 14-pg., 2024-10-01.

Abstract

Anthocyanins have antioxidant, anti-inflammatory, and anticancer properties but have limited bioaccessibility and bioavailability due to molecular instability in the gastrointestinal tract. This study evaluated the absorption and biodistribution of free and nanoencapsulated radiolabeled anthocyanin (cyanidin-3-O-glucoside). A new methodology was efficiently developed for radiolabeling anthocyanins with Technetium (99mTc-anthocyanins). Then, the anthocyanins were nanoencapsulated through self-assembly using citrus pectin and lysozyme. The nanostructures have a size of 190 nm, a zeta potential of -30 mV, and an invariably spherical and homogeneous morphology. The biodistribution in different tissues, the kinetics of absorption, and molecular visualization by micro single-photon emission computed tomography/computed tomography (mu SPECT/CT) showed that the nanoencapsulated anthocyanins are absorbed differently than free anthocyanin in mice. After oral administration, nanostructured anthocyanins were delivered to the blood, spleen, bladder, pancreas, and bone, unlike unencapsulated anthocyanins found only in kidneys and bladder. In silico data indicated the stabilization between compounds in nanocapsules and demonstrated the pH-dependent release of anthocyanins in the intestine. The nanoencapsulation alters the absorption kinetics, increasing the blood's bioavailability and the organs' uptake, suggesting an improvement of the biological effects and potential clinical application. (AU)

• FAPESP's process: 13/07914-8 - FoRC - Food Research Center
• Grantee: Bernadette Dora Gombossy de Melo Franco
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 22/12834-2 - Effects of modified pectins on gut health: pre-clinical and clinical studies to assess cellular, transcriptional, and epigenetic effects
• Grantee: Joao Paulo Fabi
• Support Opportunities: Regular Research Grants
• FAPESP's process: 23/09620-3 - Nanocarriers of Pectin and Lysozyme-Based Anthocyanins: Experimental Approach to Assess Increased Bioavailability and Anticancer Effects in the Colon of Rats
• Grantee: Thiécla Katiane Osvaldt Rosales
• Support Opportunities: Scholarships in Brazil - Support Program for Fixating Young Doctors
• FAPESP's process: 23/01396-7 - Pectin and Lysozyme-Based Anthocyanin Nanocarriers: Experimental Approach to Assess Bioavailability Improvement and Anticancer Effects in the Colon of Rats
• Grantee: Joao Paulo Fabi
• Support Opportunities: Regular Research Grants