Synthesis and physical-chemical characterization of chitosan grafted with hyaluronic acid as potential application in biomaterials

Author(s)

de Souza, Winicius Ruis ; Quevedo, Bruna V. ; Garcia, Pamela Soto ; da Silva, Lucas Camargo Soares Carvalho ; Komatsu, Daniel ; Duek, Eliana Aparecida de Rezende

• Document type: Journal article
• Source: POLYMER BULLETIN; v. N/A, p. 19-pg., 2024-12-04.

Abstract

Chitosan (Chit) is a naturally derived polymer with significant potential for applications in medicine and pharmacology. Despite its biological activity, biocompatibility, cost-effectiveness, biodegradability, and low toxicity, Chit limited water solubility at pH < 6, restricts its use in biological fields, especially in medicine. To overcome this limitation, the study proposes the graphitization of Chit with hyaluronic acid (HA) (Chit-HA). The graphitization was confirmed by Fourier Transform Infrared Spectroscopy (FTIR) and Hydrogen Nuclear Magnetic Resonance Spectroscopy (1H NMR) analysis. In this work the grafting yield percentage was 75.2%. Thermogravimetric Analysis (TGA) showed an increasing stage of mass loss for Chit-HA, attributed to the decomposition of HA in the Chit chain. Differential Scanning Calorimetry (DSC) revealed a new exothermic peak at 256.9 degrees C for Chit-HA, indicating the thermal degradation of HA grafted onto the Chit chain. Solubility assay demonstrated an increase in water solubility for Chit-HA compared to Chit. In contrast to the Chit sample, the Zeta Potential measurements of Chit-HA exhibited negative charge throughout the analyzed pH range. Therefore, the results confirm the successful grafting of Chit with HA, resulting in a new material with potential application in the biomedical field. (AU)

• FAPESP's process: 23/17083-8 - Development and Evaluation of Injectable Bioactive Hydrogel Composed of Silk Fibroin, Puerarin and Reduced Graphene Oxide Directed for Post-Myocardial Infarction Therapy
• Grantee: Bruna Vanessa Quevedo Miranda
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 20/09917-8 - Rheological analysis of bioactive gel based on silk fibroin/PLDLA/simvastatin for 3D printing
• Grantee: Eliana Aparecida de Rezende Duek
• Support Opportunities: Regular Research Grants