In vitro photoprotective efficacy and photostability of synthesized star-shaped ZnO nanoaggregates associated with ethylhexyl methoxycinnamate and butyl methoxydibenzoylmethane

Author(s)

Machado, Gustavo Teixeira ; Chiabai, Caio Rui ; Pinheiro, Mariana Santos ; Pinto, Claudineia Aparecida Sales de Oliveira ; Baby, Andre Rolim ; Andrade, George Ricardo Santana ; Pessoa, Fabiana Vieira Lima Solino

• Document type: Journal article
• Source: JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY; v. 261, p. 8-pg., 2024-11-29.

Abstract

The heightened susceptibility to skin cancer correlates with exposure to ultraviolet (UV) radiation, which can induce various cutaneous injuries. Inorganic UV filters, like zinc oxide (ZnO), are extensively utilized in sunscreens owing to their capacity to scatter and reflect UV radiation. The efficacy of inorganic UV filters can be augmented across a wider spectrum through synergistic combinations with other active compounds, such as organic UV filters. In this study, we synthesized and evaluated the in vitro effectiveness of star-shaped ZnO nanoaggregates. The samples were obtained employing a simple and greener precipitation method in an aqueous solution. The synthesized ZnO nanoaggregates were characterized through X-ray diffraction, UV/Vis spectroscopy, and transmission electron microscopy. The characterization suggests the aggregation of nanocrystals, with hexagonal wurtzite geometry, enabling the formation of star-shaped particles. The irregular (non-flat) surface and high surface area combined with light absorption in the UVA region make the material susceptible to the application of sun protection. Sunscreens formulated with the synthesized ZnOng in conjunction with ethylhexyl p-methoxycinnamate (EHMC) and/or butyl methoxydibenzoylmethane (BMDBM) were evaluated for in vitro sun protection factor (SPF) and critical wavelength (c lambda) using diffuse reflectance spectrophotometry with an integrated sphere. Photostability assessments were also conducted using artificial UV radiation. Sunscreen formulations containing ZnOng in combination with EHMC and BMDBM exhibited a substantial increase in in vitro SPF, approximately 990 % (from SPF 26 to 285). Furthermore, the synthesized ZnOng demonstrated higher in vitro efficacy compared to commercial ZnO active ingredients. Although all samples experienced reductions in SPF values during the photostability assay, ZnOng + EHMC + BMDBM retained a broad-spectrum profile (SPF > 15 and c lambda > 370 nm). Based on the distinctive properties and in vitro performance of star-shaped ZnOng, we propose that this synthesized inorganic UV filter could serve as an alternative for enhancing the SPF of sunscreen systems while reducing the concentrations of organic UV filters. (AU)

• FAPESP's process: 19/16169-0 - Ex vivo and in vivo establishment of new chemopreventive properties of UV filters
• Grantee: André Rolim Baby
• Support Opportunities: Regular Research Grants
• FAPESP's process: 24/01920-0 - Development and application of an inclusive protocol to prove the effectiveness of active sunscreen ingredients for the skin health of black individuals
• Grantee: André Rolim Baby
• Support Opportunities: Regular Research Grants