Annotation of GC-MS Data of Antimicrobial Constituents in the Antarctic Seaweed Phaeurus antarcticus by Molecular Networking

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dos Santos, Gustavo Souza ; Teixeira, Maria Valdeline Sousa ; Clementino, Leandro da Costa ; Gama-Filho, Pericles Abreu ; Pereira, Luiz Miguel ; Teixeira, Thaiz Rodrigues ; Furtado, Niege Aracari Jacometti Cardoso ; Graminha, Marcia Aparecida da Silva ; Yatsuda, Ana Patricia ; Neto, Pio Colepicolo ; Edrada-Ebel, Ru Angelie ; Debonsi, Hosana Maria

• Document type: Journal article
• Source: CHEMISTRY & BIODIVERSITY; v. 20, n. 12, p. 11-pg., 2023-11-20.

Abstract

Phaeurus antarcticus is a member of the Desmarestiaceae family endemic to the Antarctic Peninsula. Reports addressing its chemical composition and biological activities are scarce. Herein, bioactive non-polar compounds of P. antarcticus against pathogenic bacteria, Leishmania amazonensis and Neospora caninum parasites were targeted through GC-MS Molecular Networking and multivariate analysis (OPLS-DA). The effects on horseradish peroxidase (HRP) were also evaluated. P. antarcticus exhibited selective bacteriostatic and bactericidal activities against Staphylococcus aureus with MIC and MBC values from 6.25-100 mu g mL(-1). Fractions HX-FC and HX-FD were the most active against L. amazonensis with EC50 ranging from 18.5-62.3 mu g mL(-1). Additionally, fractions HX-FC and HX-FD showed potent inhibition of N. caninum at EC50 values of 2.8 and 6.3 mu g mL(-1), respectively. All fractions inhibited HRP activity, indicating possible interactions with Heme proteins. It was possible to annotate compounds from tree mains clusters, containing terpenoids, steroids, fatty acids, and alcohols by correlating the spectral data of the GC-MS analysis with Molecular Networking and the OPLS-DA results. (AU)

• FAPESP's process: 16/06931-4 - Biodiversity of marine algae and metabolites of economical impact
• Grantee: Pio Colepicolo Neto
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 14/50926-0 - INCT 2014: biodiversity and natural products
• Grantee: Vanderlan da Silva Bolzani
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants
• FAPESP's process: 17/03552-5 - Leishmaniasis: from screening to the study of mechanisms of action, a contribution to the discovery of new bioactive molecules
• Grantee: Marcia Aparecida Silva Graminha
• Support Opportunities: Regular Research Grants