Protective effect of bisabolol on glucose metabolism in salivary gland of male diabetic rats

Author(s)

Santos, Leticia Martins ; Sipert, Carla Renata ; dos Santos, Gabriel Soares ; dos Santos, Gabriella Soares ; Dionisio, Thiago Jose ; dos Santos, Carlos Ferreira ; de Souza, Douglas Nesadal ; Nogueira, Fernando Neves

• Document type: Journal article
• Source: ARCHIVES OF ORAL BIOLOGY; v. 174, p. 9-pg., 2025-06-01.

Abstract

Objective: This study aimed to evaluate the effect of bisabolol treatment on the activity and expression of the main enzymes of the glycolytic pathway in the submandibular and parotid salivary glands of Streptozotocin (STZ)-induced diabetic rats. Design: Twenty-four male Wistar rats, aged 60 days, were divided into four groups: Control, diabetic, control+Bisabolol, and diabetic+Bisabolol. A single intraperitoneal injection of Streptozotocin induced diabetes. The treated group received a daily dose of alpha-Bisabolol (25 mg/kg of body weight dissolved in 0.1 mL of sunflower oil) by gavage for 21 days. The untreated group received only the vehicle. All animals were euthanized 28 days after induction, and the parotid and submandibular glands were removed, frozen in liquid nitrogen, and stored at -80 degrees C. The expression of the main enzymes of the glycolytic pathway was analyzed through RT-qPCR. Biochemical analyses were conducted to verify Hexokinase (HK), Phosphofructokinase (PFK), Pyruvate kinase (PK), and Lactate dehydrogenase (LDH) activity. The data were statistically compared via the Student t-test and analysis of variance (ANOVA) followed by Tukey's test, where results were considered statistically significant at p < 0.05. Results: In general, bisabolol restored the HK and PFK activities and also restored Hexokinase 2 (Hk2), Phosphofructokinase muscle isoform (Pfkm) and Phosphofructokinase platelet isoform (Pfkp) expression in the bisabolol-treated diabetic rats. The LDH activity increased in diabetic rats with bisabolol. Conclusion: Bisabolol treatment restored the activity and expression of key glycolytic enzymes, suggesting that it may have therapeutic potential in mitigating the salivary glands metabolic dysregulation caused by diabetes in rats. (AU)

• FAPESP's process: 20/12726-0 - Role of osteoprotegerin on in vitro modulation of monocytes by apical papilla cells
• Grantee: Carla Renata Sipert
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/03965-2 - Role of the renin-angiotensin system in different oral inflammatory models: an experimental interdisciplinary and clinical approach
• Grantee: Carlos Ferreira dos Santos
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 19/14556-7 - Effect of diabetes and insulin on the production and activity of key enzymes of energetic metabolism of the submandibular gland of rats
• Grantee: Fernando Neves Nogueira
• Support Opportunities: Regular Research Grants
• FAPESP's process: 22/14686-0 - Role of osteoprotegerin on in vitro modulation of monocytes by apical papilla cells
• Grantee: Letícia Martins Santos
• Support Opportunities: Scholarships in Brazil - Doctorate