Ceftriaxone-resistant Salmonella Panama ST48 detected in poultry food chain: A phylogeographical analysis

Author(s)

Fontana, Herrison ; Fuga, Bruna ; Martins-Goncalves, Thais ; Esposito, Fernanda ; Cardoso, Brenda ; Beatriz, Laura Rodrigues ; Lincopan, Nilton

• Document type: Journal article
• Source: ONE HEALTH; v. 20, p. 5-pg., 2025-06-01.

Abstract

Salmonella Panama is a clinically relevant serovar isolated from food sources, particularly poultry and swine, being also reported in human gastrointestinal and extraintestinal disease globally. The emergence of broadspectrum cephalosporin-resistant Salmonella has been considered a One Health matter that demands continuous microbiologic and genomic surveillance for a comprehensive understanding and mitigation actions. During a local surveillance study conducted to monitoring WHO priority Salmonella spp. in the Brazilian poultry food chain, a ceftriaxone-resistant Salmonella strain (PN2) exhibiting a positive extended-spectrum beta-lactamase (ESBL) phenotype was recovered from a chicken sample, in a slaughterhouse under federal inspection. Clinically relevant genomic data confirmed Salmonella Panama sequence type (ST) 48 carrying the blaCTX-M-8 ESBL gene into the broad-host range IncM1conjugative plasmid, and displaying point mutations in the quinolone determining resistance region (QRDR), related to fluoroquinolone non-susceptibility. Phylogeographical analysis of publicly available global genomes of S. Panama ST48 revealed a potential concern for One Health issues due to its zoonotic nature, clustering PN2 (52-153 cgSNP differences) along with genomically related S. Panama strains isolated from human infections, food products, and farm animals, in the United States of America and Brazil. Interestingly, using Bayesian clustering method, the PN2 strain was grouped in the main clade C4, along with a CTX-M-55-producing S. Panama strain isolated from a human stool sample in Taiwan. In summary, we alert for the potential risk of dissemination of a neglected serovar that has contributed to the Salmonella global disease burden through the food supply chain. (AU)

• FAPESP's process: 24/19006-3 - Genome-wide association studies of critical priority pathogens integrated to One Health in Brazil
• Grantee: Herrison Yoshiki Yocida Fontana
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 20/08224-9 - OneBR (One Health Brazilian Resistance): integrated genomic database for surveillance, diagnosis, management and treatment of antimicrobial resistance in the human-animal-environment interface, in Brazil
• Grantee: Nilton Erbet Lincopan Huenuman
• Support Opportunities: Regular Research Grants
• FAPESP's process: 24/20180-8 - WildBR: platform for epidemiological and genomic surveillance of bacterial and fungal resistance in Brazilian wildlife
• Grantee: Thais Martins Gonçalves
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 19/15578-4 - Virulome and pathogenicity of carbapenem- and polymyxin-resistant priority pathogens
• Grantee: Fernanda Ribeiro dos Santos Esposito
• Support Opportunities: Scholarships in Brazil - Doctorate