Salmonella Minnesota sequence type 548 harbouring a type 2 IncC megaplasmid of antimicrobial resistance and virulence (pESM) infecting a companion animal

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Sartori, Luciana ; Furlan, Joao Pedro Rueda ; Sellera, Fabio P. ; Barbosa, Fernanda B. ; Fuga, Bruna ; Melocco, Gregory Batista ; Dantas, Karine Sousa ; Martins-Gonsalves, Thais ; Tavella, Ronan Adler ; Gales, Ana Cristina ; Lincopan, Nilton ; Knobl, Terezinha

• Document type: Journal article
• Source: JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE; v. 44, p. 3-pg., 2025-07-26.

Abstract

Objectives: This study reports the identification and genomic characteristics of Salmonella strains isolated from a blood sample of a 9-year-old male Persian cat with a systemic infection (strain M885) and from a pleural effusion sample of a 9-year-old male Bulldog (strain T886) in Brazil. Methods: Genomic DNA was sequenced using the Illumina NextSeq platform, de novo assembled by SPAdes version 3.15.2, and annotated by the RAST server. Serovars, sequence types (STs), antimicrobial resistance genes, plasmid replicons, Salmonella pathogenicity islands, and single nucleotide polymorphism (SNP) analysis were accomplished by bioinformatic tools. Results: Strains M885 and T886 belonged to the serovars Sandiego (ST126) and Minnesota (ST548), respectively. Strain T886 carried blaCMY-2 and qnrB19 onto IncC2 and Col(pHAD28) plasmids, respectively. The blaCMY-2 -bearing IncC2 plasmid also harboured mercury tolerance genes and the yersiniabactin virulence gene cluster, being classified as a type 2 IncC megaplasmid of antimicrobial resistance and virulence (plasmid for emergent S . Minnesota [pESM]). SNP-based analysis revealed clonal relatedness between the T886 strain and a CMY-2-producing S . Minnesota ST548 previously isolated from chicken sausage in Brazil, supporting a common ancestral origin. Conclusions: This study underscores the importance of monitoring S. enterica as the causative agent of extra-intestinal infections in small animal medicine. Therefore, the transmission dynamics and effective strategies for managing infections produced by multidrug-resistant clones adapted to the human-animalenvironmental interface warrant further investigation. (c) 2025 The Author(s). Published by Elsevier Ltd on behalf of International Society for Antimicrobial ( http://creativecommons.org/licenses/by-nc-nd/4.0/ ) (AU)

• FAPESP's process: 21/10599-3 - The Antimicrobial Resistance Institute of São Paulo (The Aries Project)
• Grantee: Arnaldo Lopes Colombo
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
• FAPESP's process: 20/08224-9 - OneBR (One Health Brazilian Resistance): integrated genomic database for surveillance, diagnosis, management and treatment of antimicrobial resistance in the human-animal-environment interface, in Brazil
• Grantee: Nilton Erbet Lincopan Huenuman
• Support Opportunities: Regular Research Grants
• FAPESP's process: 22/11917-1 - Genomic analysis of pandemic lineages of Avian Pathogenic Escherichia coli (APEC) focusing surveillance of Avian Colibacillosis in Brazil
• Grantee: Terezinha Knöbl
• Support Opportunities: Regular Research Grants