Use of Carnobacterium maltaromaticum cultures and hydroalcoholic extract of Lippia sidoides Cham. against Listeria monocytogenes in fish model systems

Author(s)

dos Reis, Fernanda B. ^[1] ; de Souza, Vanessa M. ^[1] ; Thomaz, Marta R. S. ^[1] ; Fernandes, Luciana P. ^[1] ; de Oliveira, Wanderley P. ^[1] ; De Martinis, Elaine C. P. ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Sao Paulo - Brazil

• Document type: Journal article
• Source: International Journal of Food Microbiology; v. 146, n. 3, p. 228-234, APR 29 2011.
• Web of Science Citations: 18

Abstract

Minimally processed refrigerated ready-to-eat fishes may offer health risk of severe infection to susceptible individuals due to contamination by the psychrotolerant bacterium L monocytogenes. In this work, inhibition of L monocytogenes by a plant extract and lactic acid bacteria (IAB) was studied in model fish systems kept at 5 degrees C for 35 days. For that, fillets of tropical fish ``surubim{''} (Pseudoplatystoma sp.) and hydroalcoholic extract of the plant Lippia sidoides Cham. ({''}alecrim pimenta{''}) were used. Fish peptone broth (FPB), ``surubim{''} broth and ``surubim{''} homogenate were inoculated with combinations of L monocytogenes and bacteriocin-producing Carnobacterium maltaromaticum (C2 and A9b(+)) and non bacteriocin-producing C. maltaromaticum (A9b(-)), in the presence or absence of extract of ``alecrim pimenta{''} (EAP). In all model systems, monocultures of L monocytogenes and carnobacteria reached final populations >= 10(8) CFU/ml after 35 days, except for L monocytogenes in ``surubim{''} homogenate (10(4) CFU/ml). In FPB, EAP alone and combined with cultures of LAB inhibited L monocytogenes but carnobacteria without EAP were only weakly antilisterial. In ``surubim{''} broth, EAP alone did not prevent L monocytogenes growth but cultures of carnobacteria combined or not with EAP inhibited L monocytogenes, with more pronounced effect being observed for C maltaromaticum C2, which produced bacteriocin. In ``surubim{''} homogenate, EAP alone and combined with cultures of C. maltaromaticum A9b(-) and A9b(+) were strongly inhibitory to L monocytogenes, while C maltaromaticum C2 with EAP caused transient inhibition of L monocytogenes. No significant inhibition of L monocytogenes was observed for carnobacteria in ``surubim{''} homogenate without EAP. In conclusion, it was observed that the use of EAP and cultures of carnobacteria have potential to inhibit L monocytogenes in fish systems and the applications should be carefully studied, considering the influence of food matrix. (c) 2011 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 07/05995-0 - Biopreservation of fish (surubim Pseudoplatystoma sp) with use of the bacteriocinogenic lactic acid bacteria (Carnobacterium piscicola C2) and plant extracts of rosemary pepper (Lippia sidoides Cham.)
• Grantee: Fernanda Barbosa dos Reis Teixeira
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 09/08110-4 - Biopreservation of Surubim fish (Pseudoplatystoma sp.) by use of bacteriocinogenic lactic acid bacterium (Carnobacterium piscicola C2) and extracts of pepper rosemary (Lippia sidoides Cham.)
• Grantee: Elaine Cristina Pereira de Martinis
• Support Opportunities: Regular Research Grants