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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Use of Carnobacterium maltaromaticum cultures and hydroalcoholic extract of Lippia sidoides Cham. against Listeria monocytogenes in fish model systems

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dos Reis, Fernanda B. [1] ; de Souza, Vanessa M. [1] ; Thomaz, Marta R. S. [1] ; Fernandes, Luciana P. [1] ; de Oliveira, Wanderley P. [1] ; De Martinis, Elaine C. P. [1]
Total Authors: 6
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14040903 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: International Journal of Food Microbiology; v. 146, n. 3, p. 228-234, APR 29 2011.
Web of Science Citations: 18

Minimally processed refrigerated ready-to-eat fishes may offer health risk of severe infection to susceptible individuals due to contamination by the psychrotolerant bacterium L monocytogenes. In this work, inhibition of L monocytogenes by a plant extract and lactic acid bacteria (IAB) was studied in model fish systems kept at 5 degrees C for 35 days. For that, fillets of tropical fish ``surubim{''} (Pseudoplatystoma sp.) and hydroalcoholic extract of the plant Lippia sidoides Cham. ({''}alecrim pimenta{''}) were used. Fish peptone broth (FPB), ``surubim{''} broth and ``surubim{''} homogenate were inoculated with combinations of L monocytogenes and bacteriocin-producing Carnobacterium maltaromaticum (C2 and A9b(+)) and non bacteriocin-producing C. maltaromaticum (A9b(-)), in the presence or absence of extract of ``alecrim pimenta{''} (EAP). In all model systems, monocultures of L monocytogenes and carnobacteria reached final populations >= 10(8) CFU/ml after 35 days, except for L monocytogenes in ``surubim{''} homogenate (10(4) CFU/ml). In FPB, EAP alone and combined with cultures of LAB inhibited L monocytogenes but carnobacteria without EAP were only weakly antilisterial. In ``surubim{''} broth, EAP alone did not prevent L monocytogenes growth but cultures of carnobacteria combined or not with EAP inhibited L monocytogenes, with more pronounced effect being observed for C maltaromaticum C2, which produced bacteriocin. In ``surubim{''} homogenate, EAP alone and combined with cultures of C. maltaromaticum A9b(-) and A9b(+) were strongly inhibitory to L monocytogenes, while C maltaromaticum C2 with EAP caused transient inhibition of L monocytogenes. No significant inhibition of L monocytogenes was observed for carnobacteria in ``surubim{''} homogenate without EAP. In conclusion, it was observed that the use of EAP and cultures of carnobacteria have potential to inhibit L monocytogenes in fish systems and the applications should be carefully studied, considering the influence of food matrix. (c) 2011 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 07/05995-0 - Biopreservation of fish (surubim Pseudoplatystoma sp) with use of the bacteriocinogenic lactic acid bacteria (Carnobacterium piscicola C2) and plant extracts of rosemary pepper (Lippia sidoides Cham.)
Grantee:Fernanda Barbosa dos Reis Teixeira
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 09/08110-4 - Biopreservation of Surubim fish (Pseudoplatystoma sp.) by use of bacteriocinogenic lactic acid bacterium (Carnobacterium piscicola C2) and extracts of pepper rosemary (Lippia sidoides Cham.)
Grantee:Elaine Cristina Pereira de Martinis
Support Opportunities: Regular Research Grants