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Extracts from Eugenia uniflora L. and Lippia sidoides Cham. leaves by sequential extraction in fixed bed using supercritical CO2, ethanol and water as solvents

Grant number: 11/14309-8
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2012
Effective date (End): July 31, 2013
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Engineering
Principal Investigator:Fernando Antonio Cabral
Grantee:Tábata Tayara Garmus
Host Institution: Faculdade de Engenharia de Alimentos (FEA). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

Leaf extracts from Eugenia uniflora and from Lippia sidoides will be obtained by sequential extraction in fixed bed, first by extraction with supercritical carbon dioxide (scCO2) at 60º C and 400 bar, followed by extraction with ethanol at 60° C and, finally followed by extraction with water at 60º C. Extraction curves (mass versus time extract) will be obtained in order to evaluate the performance of extraction on the yield and concentration of phenol as a function of time and type of the solvent. The essential oil will be obtained in the first step with scCO2 and, it will be evaluated as chemical profile by GC-MS and subsequent statements will be evaluated on the content of total phenols and flavonoids. For comparison, two types of aqueous and ethanol extracts will be obtained: 1) Single-Stage Extracts from the leaves with ethanol fixed bed at 60° C without prior extraction with water and scCO2 fixed bed at 60° C without prior extraction and 2) extracts by following the conventional method reported in the literature and used in earlier work, not in a fixed bed.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GARMUS, TABATA T.; PAVIANI, LOSIANE C.; QUEIROGA, CARMEN L.; CABRAL, FERNANDO A.. Extraction of phenolic compounds from pepper-rosmarin (Lippia sidoides Cham.) leaves by sequential extraction in fixed bed extractor using supercritical CO2, ethanol and water as solvents. JOURNAL OF SUPERCRITICAL FLUIDS, v. 99, p. 68-75, . (11/14309-8)
GARMUS, TABATA T.; PAVIANI, LOSIANE C.; QUEIROGA, CARMEN L.; MAGALHAES, PEDRO M.; CABRAL, FERNANDO A.. Extraction of phenolic compounds from pitanga (Eugenia uniflora L.) leaves by sequential extraction in fixed bed extractor using supercritical CO2, ethanol and water as solvents. JOURNAL OF SUPERCRITICAL FLUIDS, v. 86, p. 4-14, . (11/14309-8)

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