| Full text | |
| Author(s): |
Pucca, Manuela B.
;
Peigneur, Steve
;
Cologna, Camila T.
;
Cerni, Felipe A.
;
Zoccal, Karina F.
;
Bordon, Karla de C. F.
;
Faccioli, Lucia H.
;
Tytgat, Jan
;
Arantes, Eliane C.
Total Authors: 9
|
| Document type: | Journal article |
| Source: | Biochimie; v. 115, p. 9-pg., 2015-08-01. |
| Abstract | |
Tityus serrulatus (Ts) venom is composed of mainly neurotoxins specific for voltage-gated K+ and Na+ channels, which are expressed in many cells such as macrophages. Macrophages are the first line of defense invasion and they participate in the inflammatory response of Ts envenoming. However, little is known about the effect of Ts toxins on macrophage activation. This study investigated the effect of Ts5 toxin on different sodium channels as well as its role on the macrophage immunomodulation. The electrophysiological assays showed that Ts5 inhibits the rapid inactivation of the mammalian sodium channels Nav1.2, Nav1.3, Nav1A, Nav1.5, Nav1.6 and Nav1.7. Interestingly, Ts5 also inhibits the inactivation of the insect Drosophila melanogaster sodium channel (DmNav1), and it is therefore classified as the first Ts a-like toxin. The immunological experiments on macrophages reveal that Ts5 is a proinflammatory toxin inducing the cytokine production of tumor necrosis factor (TNF)-alpha and interleukin (IL)-6. On the basis of recent literature, our study also stresses a possible mechanism responsible for venom-associated molecular patterns (VAMPS) internalization and macrophage activation and moreover we suggest two main pathways of VAMPs signaling: direct and indirect. This work provides useful insights for a better understanding of the involvement of VAMPs in macrophage modulation. (C) 2015 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved. (AU) | |
| FAPESP's process: | 14/03332-7 - Study of the relationship between inflammasome activation and lipid mediators production with pulmonary inflammation induced by scorpion venom with and without hyaluronidase |
| Grantee: | Karina Furlani Zoccal |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |
| FAPESP's process: | 13/21342-7 - Electrophysiological characterization of Ts19 fragment II, a new toxin purified from Tityus serrulatus venom |
| Grantee: | Felipe Augusto Cerni |
| Support Opportunities: | Scholarships abroad - Research Internship - Doctorate |
| FAPESP's process: | 13/21329-0 - Electrophysiological characterization of Tityus serrulatus venom toxins Ts3, Ts4, Ts6, Ts8, Ts15 and TS venom peptide 7: looking for new desired drugs |
| Grantee: | Manuela Berto Pucca |
| Support Opportunities: | Scholarships abroad - Research Internship - Post-doctor |
| FAPESP's process: | 09/07169-5 - Lipid mediators as regulators of immune response |
| Grantee: | Lúcia Helena Faccioli |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 13/26200-6 - Venomics Plataform of Pachycondyla villosa Proteome, Transcriptome, Cloning and Expression of toxins with biotechnological potential |
| Grantee: | Camila Takeno Cologna |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |