Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genotoxic effects of (-)-cubebin in somatic cells of mice

Full text
Author(s):
Maistro, Edson Luis [1] ; Marcondes Natel, Andre Vinicius [1] ; Bianco de Souza, Gustavo Henrique [2] ; Perazzo, Fabio Ferreira [3]
Total Authors: 4
Affiliation:
[1] Univ Estadual Paulista, UNESP, Fac Filosofia & Ciencias, Dept Fonoaudiol, BR-17525900 Marilia, SP - Brazil
[2] Univ Fed Ouro Preto, Escola Farm, Lab Farmacognosia, BR-35400000 Ouro Preto, MG - Brazil
[3] Univ Fed Sao Paulo, Unifesp, Dept Ciencias Exatas & Terra, BR-09972270 Diadema, MG - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF APPLIED TOXICOLOGY; v. 31, n. 2, p. 185-189, MAR 2011.
Web of Science Citations: 7
Abstract

(-)-Cubebin belongs to the dibenzylbutyrolactone lignan group, which is widely distributed in the plant kingdom. Because this compound shows interesting biological activities, it is extremely important to evaluate its possible genotoxic activity to allow its safe use in humans. Thus, the present study was performed to investigate the genotoxicity potential activity of (-)-cubebin assessed by two assays: micronucleus in bone marrow cells and comet test in peripheral blood leukocytes of Swiss mice. In the (-)-cubebin dose range-finding assays, the maximum tolerated dose was greater than 2000 mg kg(-1). The compound was administered by an oral route at single doses of 250, 500 and 2000 mg kg(-1) body weight. Cytotoxicity was assessed by scoring 200 consecutive total polychromatic (PCE) and normochromatic (NCE) erythrocytes (PCE/NCE ratio). Under our experimental conditions, micronucleus and comet assays, respectively, showed that (-)-cubebin caused dose-related clastogenic and genotoxic effects in the somatic cells investigated. PCE/NCE ratio showed no cytotoxicity for the three doses of the compound. The data suggest caution in the ingestion of (-)-cubebin by humans, especially at high doses. Copyright (C) 2010 John Wiley \& Sons, Ltd. (AU)