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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Isolation and analysis of bioactive isoflavonoids and chalcone from a new type of Brazilian propolis

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Author(s):
Oldoni, Tatiane Luiza C. [1] ; Cabral, Ingridy S. R. [1] ; d'Arce, Marisa A. B. Regitano [1] ; Rosalen, Pedro L. [2] ; Ikegaki, Masaharu [3] ; Nascimento, Andrea M. [1] ; Alencar, Severino M. [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Agri Food Ind Food & Nutr, BR-13418900 Piracicaba, SP - Brazil
[2] Univ Estadual Campinas, UNICAMP, Piracicaba Dent Sch, Dept Physiol Sci, BR-13414903 Piracicaba, SP - Brazil
[3] Univ Fed Alfenas, Fac Pharmaceut Sci, BR-37130000 Alfenas, MG - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Separation and Purification Technology; v. 77, n. 2, p. 208-213, FEB 20 2011.
Web of Science Citations: 43
Abstract

Activity-directed fractionation and purification processes were employed to identify isoflavonoids with antioxidant and antimicrobial activities from Brazilian red propolis. Crude propolis was extracted with ethanol (80%. v/v) and fractioned by liquid-liquid extraction technique using hexane and chloroform. Since chloroform fraction showed strong antioxidant and antimicrobial activities it was purified and isolated using various chromatographic techniques. Comparing our spectral data (UV, NMR, and mass spectrometry) with values found in the literature, we identified two bioactive isoflavonoids (vestitol and neovestitol), together with one chalcone (isoliquiritigenin). Vestitol presented higher antioxidant activity against beta-carotene consumption than neovestitol. The antimicrobial activity of these three compounds against Staphylococcus aureus, Streptococcus mutans, and Actinomyces naeslundii was evaluated and we concluded that isoliquiritigenin was the most active one with lower MIC, ranging from 15.6 to 62.5 mu g/mL. Our results showed that Brazilian red propolis has biologically active isoflavonoids that may be used as a mild antioxidant and antimicrobial for food preservation. (C) 2010 Elsevier B.V. All rights reserved. (AU)