Diversity of Pulsed-Field Gel Electrophoresis Patterns of Mycobacterium abscessus Type 2 Clinical Isolates

Author(s)

Matsumoto, Cristianne Kayoko ^[1] ; Chimara, Erica ^[2] ; Bombarda, Sidney ^[3] ; Duarte, Rafael Silva ^[4] ; Leao, Sylvia Cardoso ^[1]

Affiliation

^[1] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, BR-04023062 Sao Paulo - Brazil

^[2] Inst Adolfo Lutz Registro, Setor Micobacterias, Sao Paulo - Brazil

^[3] Ctr Vigilancia Epidemiol Prof Alexandre Vranjac, Div TB, Sao Paulo - Brazil

^[4] Univ Fed Rio de Janeiro, Inst Microbiol, BR-21941 Rio De Janeiro - Brazil

• Document type: Journal article
• Source: Journal of Clinical Microbiology; v. 49, n. 1, p. 62-68, JAN 2011.
• Web of Science Citations: 19

Abstract

An epidemic of infections by rapidly growing mycobacteria related to surgical procedures between 2004 and 2008 in Brazil was caused by a unique strain showing the Mycobacterium abscessus type 2 pattern when it was analyzed by the molecular method of PCR-restriction enzyme analysis of the hsp65 gene (PRA-hsp65). In order to investigate the diversity of M. abscessus type 2 clinical isolates and to assess whether this epidemic strain was present in specimens from nonsurgical patients, we studied 52 isolates from 38 patients showing this characteristic PRA-hsp65 pattern obtained between 2005 and 2009. All isolates were identified by sequencing of region V of the rpoB gene and typed by pulsed-field gel electrophoresis (PFGE) using two restriction enzymes, DraI and AseI. Seven isolates obtained from sputum, bronchoalveolar lavage fluid, and urine in three different Brazilian states showed rpoB sequences 100% similar to the rpoB sequence of epidemic strain INCQS 594 and PFGE patterns highly related to the patterns of isolates, evidencing the presence of the epidemic strain in isolates from patients not associated with the surgical epidemic. The remaining isolates showed diverse rpoB sequences, with the highest similarities being to the corresponding sequences of M. massiliense(T) CIP 108297 (21 isolates), M. bolletii(T) CIP 108541 (19 isolates), or M. abscessus(T) ATCC 19977 (5 isolates). Two additional clusters could be detected by PFGE. PFGE showed 100% typeability and reproducibility and discriminatory powers, calculated by Simpson's index of diversity, of 0.978 (DraI) and 0.986 (AseI), confirming its suitability for the discrimination of M. abscessus type 2 isolates. (AU)

• FAPESP's process: 08/01451-8 - Molecular caracterization of rapidly growing micobacteria associated or not to outbreaks in laparoscopic surgeries for the development of a quick identification test
• Grantee: Cristianne Kayoko Matsumoto
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 06/01533-9 - Mycobacteria of medical significance in Brazil: molecular characterization, interaction with the environment and with macrophages
• Grantee: Sylvia Luisa Pincherle Cardoso Leão
• Support Opportunities: Research Projects - Thematic Grants