Development and characterization of 14 microsatellite loci from an enriched genomic library of Eucalyptus camaldulensis Dehnh

Author(s)

da Silva, Janete Motta ^[1] ; Barbosa de Sousa, Adna Cristina ^[2] ; de Souza, Anete Pereira ^{[2, 3]} ; Mori, Edson Seizo ^[4] ; Menezes Freitas, Miguel Luiz ^[5] ; Sebbenn, Alexandre Magno ^[5] ; Teixeira de Moraes, Mario Luiz ^{[1, 6]}

Affiliation

^[1] Univ Estadual Paulista UNESP, Fac Engn, BR-15385000 Ilha Solteira, SP - Brazil

^[2] Univ Estadual Campinas UNICAMP, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP - Brazil

^[3] Univ Estadual Campinas Unicamp, Dept Biol Vegetal, BR-13083970 Campinas, SP - Brazil

^[4] Univ Estadual Paulista UNESP, Fac Ciencias Agron, BR-18610307 Botucatu, SP - Brazil

^[5] Inst Florestal Sao Paulo, BR-01059970 Sao Paulo - Brazil

^[6] Univ Estadual Paulista UNESP, Dept Fitotecnia Tecnol Alimentos & Socioecon, Fac Engn Ilha Solteira, BR-15385000 Ilha Solteira, SP - Brazil

• Document type: Journal article
• Source: CONSERVATION GENETICS RESOURCES; v. 1, n. 1, p. 465-469, DEC 2009.
• Web of Science Citations: 6

Abstract

Eucalyptus camaldulensis Dehnh is an Australian tree species which occurs in various climatic and environmental conditions and show large genetic diversity. Twenty five microsatellite markers were developed from a CT(8)-GT(8) enriched genomic library of E. camaldulensis. The number of alleles ranged from 4 to 13 (average of 8). The polymorphism information content (PIC) and the discriminating power (D) of each primer ranged from 0.37 to 0.88 (average of 0.72) and 0.48 to 0.99 (average of 0.84), respectively. The observed and expected heterozygosities ranged from 0.28 to 0.84 and 0.25 to 0.90, respectively. Four loci showed statistically significant from Hardy-Weinberg equilibrium after Bonferroni correction (P, (5%) < 0.0038). All polymorphic markers were tested for cross-amplification in 25 different Eucalyptus species. Those microsatellite loci will be useful to investigate questions of genetic diversity and structure, gene flow, mating system and ex situ genetic conservation of E. camaldulensis. (AU)

• FAPESP's process: 05/59217-2 - Genetic variability evaluation in a base population of Eucalyptus camaldulensis Dehnh. for purposes of genetic conservation and breeding
• Grantee: Janete Motta da Silva
• Support Opportunities: Scholarships in Brazil - Doctorate