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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Superficial Distribution and Identification of Antifungal/Antimicrobial Agents on a Modified Tissue Conditioner by SEM-EDS Microanalysis: A Preliminary Study

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Author(s):
Urban, Vanessa Migliorini [1] ; Seo, Rosangela Seiko [2] ; Giannini, Marcelo [3] ; Galvao Arrais, Cesar Augusto [4]
Total Authors: 4
Affiliation:
[1] Univ Camilo Castelo Branco, Sch Dent, BR-08230030 Sao Paulo - Brazil
[2] Varzea Grande Sch Dent, Dept Prosthodont, Varzea Grande - Brazil
[3] Piracicaba Sch Dent, Dept Restorat Dent, Piracicaba - Brazil
[4] Univ Guarulhos, Sch Dent, Dept Operat Dent, Guarulhos - Brazil
Total Affiliations: 4
Document type: Journal article
Source: JOURNAL OF PROSTHODONTICS-IMPLANT ESTHETIC AND RECONSTRUCTIVE DENTISTRY; v. 18, n. 7, p. 603-610, OCT 2009.
Web of Science Citations: 16
Abstract

Purpose: This study evaluated the incorporation pattern of antifungal/antimicrobial agents added to a tissue conditioner by scanning electronmicroscopy-energy dispersive X-ray spectroscopy (SEM-EDS) analysis. Materials and Methods: The nystatin dosages incorporated into the tissue conditioner (Softone, Bosworth Co., Skokie, IL) powder were 500,000 U (G1) and 1,000,000 U (G2). The addition of miconazole was at 125 mg (G3) and 250 mg (G4), and ketoconazole was at 100 mg (G5) and 200 mg (G6). Chlorhexidine diacetate was blended at levels of 5%(G7) and 10% (G8) w/w of the total amount (6.35 g) of the tissue conditioner. The drug powder concentrations were blended with the tissue conditioner powder at different concentrations before the addition of the tissue conditioner liquid (5 mL) to the mixture. One group (G0) without any drug incorporation was used as control. Specimens (n = 5) (36 x 7 x 6 mm(3)) were plasticized at room temperature for 10 minutes and carbon sputter coated. All specimens were submitted to SEM-EDS analysis. Results: Nystatin and miconazole specimens exhibited particles with irregular shapes and sizes uniformly distributed. Ketoconazole specimens showed small spherical particles with a slight distribution throughout the matrix. Chlorhexidine specimens exhibited irregular particles up to approximately 50 mu m in size randomly dispersed within the matrix. Conclusions: Within the limitations of this in vitro study, the modified tissue conditioner showed differences in the particle distribution and size of the antifungal/antimicrobial agent added to the plasticized matrix. Further studies would discriminate the most important particle features that may influence the drug leaching from the plasticized matrix. (AU)