| Author(s): |
Grillo, Renato
[1]
;
De Melo, Nathalie F. S.
[2, 1]
;
De Araujo, Daniele R.
[2, 3]
;
De Paula, Eneida
[2]
;
Dias Filho, Newton L.
[4]
;
Rosa, Andre H.
[1]
;
Fraceto, Leonardo F.
[2, 1]
Total Authors: 7
|
| Affiliation: | [1] Univ Estadual Paulista, Dept Engenharia Ambiental, BR-18087180 Sao Paulo - Brazil
[2] Univ Estadual Campinas, Dept Bioquim, Inst Biol, Campinas, SP - Brazil
[3] Univ Fed Abc, Ctr Ciencias Humana & Nat, Santo Andre, SP - Brazil
[4] Univ Estadual Paulista, Dept Quim & Fis, Sao Paulo - Brazil
Total Affiliations: 4
|
| Document type: | Journal article |
| Source: | Latin American Journal of Pharmacy; v. 28, n. 3, p. 393-399, MAY-JUN 2009. |
| Web of Science Citations: | 16 |
| Abstract | |
Benzocaine (BZC) is an ester-type local anesthetic used mainly in topical, dermal and mucosal formulations. The present work consists of the development and validation of analytical methodology for evaluation of benzocaine content in the micro and nanoparticles produced by biodegradable polymers (polyhydroxybutyrate-co-hydroxyvalerate and poly-(L-lactide) by HPLC. The validation was done using the reversed-phase C18 column, using a mobile phase consisting of acetonitrile/water 50:50 (v/v), flow rate of 1.5 mL/min and UV-vis detector at 285 nm. The results here obtained showed that the analytical methodology is accurate, reproducible,robust and linear over the molar range concentration of 10-100 mu M of benzocaine. The limit of quantification and detection was 13.06 mu M and 3.92 mu M, respectively. The encapsulation efficiency of benzocaine In PHBV microspheres and PLA nanocapsule were 40% and 70%, respectively. The method developed was applied in the analysis of benzocaine in micro and nanoparticle systems and showed to be efficient, yielding good results. Here, this method was used to evaluate the encapsulation efficiency of benzocaine and will be used in next studies with different micro/nanoparticle formulations. (AU) | |
| FAPESP's process: | 06/00121-9 - New formulations for the controlled release of local anesthetics in dentistry: from development to clinical tests |
| Grantee: | Eneida de Paula |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 08/01222-9 - Preparation and characterization of alginate nanoparticle with bupivacaine |
| Grantee: | Renato Grillo |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |